PMID: 7529510Sep 1, 1994Paper

The effect of a hyposmotic shock on amino acid efflux from lactating rat mammary tissue: stimulation of taurine and glycine efflux via a pathway distinct from anion exchange and volume-activated anion channels

Experimental Physiology
D B ShennanD E Curran

Abstract

We have examined the effect of a hyposmotic shock, and thus cell swelling, upon the efflux of amino acids, SO4(2-) and I- from lactating mammary tissue. A hyposmotic challenge increased the efflux of taurine and glycine via a 4,4'-diisothiocyanatostilbene-2,2'- disulphonic acid (DIDS)-sensitive pathway. It appears that these amino acids do not exit via an anion-exchange mechanism following cell swelling because sulphate efflux, which uses a DIDS-sensitive exchange mechanism, was unaffected. The hyposmotic-induced efflux of taurine was not dependent upon the Na+ gradient and was not influenced by the nature of the anion in the incubation medium. In addition, taurine efflux was stimulated by incubating mammary tissue in an isosmotic medium that contained urea, suggesting that cell swelling is the stimulating factor rather than a decrease in osmolality per se. The results suggest that mammary tissue uses taurine and glycine as a means of regulating cell volume following swelling. In contrast, the efflux of glutamic acid, alanine and alpha-aminoisobutyric acid was unaffected by a hyposmotic challenge. Similarly, the efflux of I- was unaffected by such a challenge. The results suggest that volume-activated amino acid transport in la...Continue Reading

Citations

Apr 12, 2008·Purinergic Signalling·Ravshan Z Sabirov, Yasunobu Okada
Nov 23, 2006·Cell and Tissue Research·Darren LockeBarry Gusterson
Aug 19, 2007·Life Sciences·Chiun-Chien HuangPoh-Hong Lim
Jul 22, 2004·Experimental Cell Research·Darren LockeBarry Gusterson
Feb 13, 2009·Journal of Pharmacological Sciences·Eiji IsoyaYoshitaka Matsusue
Aug 18, 2006·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·David B ShennanIain F Gow
Jan 23, 2008·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·David B Shennan
May 10, 2002·Nutritional Neuroscience·Julius D Militante, John B Lombardini
Dec 11, 1997·The Journal of Experimental Zoology·E K Hoffmann
Mar 11, 1998·Acta Physiologica Scandinavica·D T Calvert, D B Shennan
Oct 17, 2013·American Journal of Physiology. Cell Physiology·D B Shennan
Jul 6, 2019·PloS One·Zhixiong Li, Mingfeng Jiang
Oct 8, 1997·The American Journal of Physiology·A StutzinF V Sepúlveda
Sep 14, 1999·The American Journal of Physiology·A StutzinF V Sepúlveda
Jul 14, 2000·Physiological Reviews·D B Shennan, M Peaker
Jan 29, 1999·Physiological Reviews·B D SchultzR J Bridges
Jun 28, 2016·NMR in Biomedicine·Markus DurstFrancesca Reineri
Mar 1, 1997·The Proceedings of the Nutrition Society·D B ShennanD T Calvert
Jan 1, 1997·Progress in Biophysics and Molecular Biology·B NiliusG Droogmans
Jan 13, 1997·Biochemical and Biophysical Research Communications·I D MillarD B Shennan
Dec 19, 2000·Biochimica Et Biophysica Acta·D B Shennan, I F Gow
Aug 26, 1998·Biochimica Et Biophysica Acta·D B ShennanV A Zammit
Sep 12, 1998·Biochimica Et Biophysica Acta·D B ShennanC A Boyd
Oct 16, 1999·Biochimica Et Biophysica Acta·I D Millar, D B Shennan
Jul 25, 1996·Biochimica Et Biophysica Acta·I D MillarD B Shennan
May 12, 2000·Biochimica Et Biophysica Acta·A C GrantD B Shennan

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