The effect of a nanothickness coating on rough titanium substrate in the osteogenic properties of human bone cells

Journal of Biomedical Materials Research. Part a
Camilla C G MouraPaulo G Coelho

Abstract

This study evaluated the effect of a bioactive ceramic coating, in the nanothickness range, onto a moderately rough surface on the osteogenic behavior of human bone cells. The cells were harvested from the mandibular mental region and were cultured over Ti-6Al-4V disks of different surfaces: as-machined (M), alumina-blasted/acid etched (AB/AE), and alumina-blasted/acid-etched + 300-500 nm thickness amorphous Ca- and P-based coating obtained by ion beam-assisted deposition (Nano). The culture was then evaluated regarding cell viability, adhesion, morphology, immunolocalization of osteopontin (OPN) and alkaline phosphatase (ALP). The results showed that the surface treatment did not interfere with cell viability. At 1 day, AB/AE and Nano showed higher adhesion than the M surface (p < 0.001). Higher adhesion was observed for the M than the Nano surface at 7 days (p < 0.005). The percentage of cells showing intracellular labeling for OPN at day 1 was significantly higher for the Nano compared to M surface (p < 0.03). The percentage of ALP intracellular labeling at 7 days was significantly higher for the AB/AE compared to the M surface (p < 0.0065); no differences were detected at 14 days. Our results suggest that the presence of a ...Continue Reading

References

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Citations

Aug 7, 2012·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Marta BaldassarriPaulo G Coelho
Jun 14, 2012·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Lukasz WitekPaulo G Coelho
Dec 8, 2011·Brazilian Oral Research·Camilla Christian Gomes MouraDarceny Zanetta-Barbosa
Aug 21, 2014·Brazilian Dental Journal·Camilla Christian Gomes MouraPriscilla Barbosa Ferreira Soares
Jun 15, 2017·Calcified Tissue International·Héllen L OliveiraEvandro Piva
Jun 12, 2017·ACS Biomaterials Science & Engineering·Guohui ShouWenjian Weng

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