The effect of acute dissociation on the electrophysiological properties of rat dorsal root ganglion neurons

Somatosensory & Motor Research
Yuanlong Song, Linlin Gao

Abstract

The acutely dissociated neurons from the dorsal root ganglia (DRGs) are extensively used. The effects of acute dissociation on the properties of these neurons are, however, not clear. In this study, the action potentials (APs) were recorded from both acutely dissociated and in vivo identified DRG neurons with patch clamp and sharp electrode recording techniques, respectively. We found that acute dissociation slowed both the depolarizing and repolarizing rate of APs, and elongated the AP duration (APD). The lower recording temperature presented in the acutely dissociated neurons contributed to about 10% of these differences. The major contributor of these differences was possibly modulation of the mRNA expression especially those of the ion channels, as suggested by our observation that acute dissociation significantly reduced the mRNA abundance of Nav1.6-1.9. In conclusion, acute dissociation altered the electrophysiological properties of the DRG neurons; the disrupted gene-expression pattern may contribute to this effect.

References

Jan 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·A ChalazonitisS M Crain
Jul 22, 1998·Proceedings of the National Academy of Sciences of the United States of America·S D Dib-HajjS G Waxman
Jun 8, 2002·Biochemical and Biophysical Research Communications·Weihong Liu, David A Saint
Feb 22, 2005·The Journal of Biological Chemistry·Man-Kyo ChungMichael J Caterina
Feb 24, 2009·Biochimica Et Biophysica Acta·Kalypso CharalambousB A Wallace
May 21, 2010·Journal of Neurophysiology·Junichi HachisukaMegumu Yoshimura
May 11, 2011·The Journal of Biological Chemistry·Luis F Lopez-SantiagoLori L Isom
Dec 3, 2016·Neural Regeneration Research·Zahir KızılayAli Yılmaz
Dec 22, 2016·Pflügers Archiv : European journal of physiology·Cindy BarbosaTheodore R Cummins
May 17, 2017·ELife·Xiulin ZhangMichael S Gold

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Citations

Jan 10, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Mayur J PatilArmen N Akopian

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