The effect of acute hypoglycemia on the cerebral NMDA receptor in newborn piglets

Brain Research
J E McGowanMaria Delivoria-Papadopoulos

Abstract

The effects of acute insulin-induced hypoglycemia on the cerebral NMDA receptor in the newborn were examined by determining [3H]MK-801 binding as an index of NMDA receptor function in 6 control and 7 hypoglycemic piglets. In hypoglycemic animals, the glucose clamp technique with constant insulin infusion was used to maintain a blood glucose concentration of 1.2 mmol/l for 120 min before obtaining cerebral cortex for further analysis; controls received a saline infusion. Concentrations of glucose, lactate, ATP, and PCr were measured in cortex, and Na+,K(+)-ATPase activity was determined in a brain cell membrane preparation. [3H]MK-801 binding was evaluated by: (1) saturation binding assays over the range of 0.5-50 nM [3H]MK-801 in the presence of 100 microM glutamate and glycine; and (2) binding assays at 10 nM [3H]MK-801 in the presence of glutamate and/or glycine at 0, 10, or 100 microM. Blood and brain glucose concentrations were significantly lower in hypoglycemic animals than controls. There was no change in brain ATP with hypoglycemia, but PCr was decreased 80% compared to control (P < 0.05). Na+,K(+)-ATPase activity was 13% lower in hypoglycemic animals (P < 0.05). Based on saturation binding data, hypoglycemia had no eff...Continue Reading

References

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Citations

Mar 15, 2006·Biology of the Neonate·Paul J Rozance, William W Hay
Sep 2, 2000·Journal of Neurosurgery·A C DuhaimeR Raghupathi
Jun 18, 2010·Early Human Development·Paul J Rozance, William W Hay
Jun 20, 2012·The European Journal of Neuroscience·Kevin E ChapmanAmy Brooks-Kayal
Feb 25, 1998·Clinical Pediatrics·L P Halamek, D K Stevenson
Jul 23, 2003·Journal of Neurochemistry·C J McDermottB J Morris
Feb 13, 2001·American Journal of Physiology. Heart and Circulatory Physiology·R A SantizoD A Pelligrino
Sep 26, 2020·Molecular Neurobiology·Igor Santana de MeloOlagide Wagner de Castro

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