The effect of adding lactic acid to canine whole blood on platelet aggregation as measured by impedance aggregometry.

Veterinary Clinical Pathology
Cheryl A Lawson, Elizabeth A Spangler

Abstract

Acidemia in sick dogs often results from the accumulation of lactic acid. The resulting decrease in blood pH can have many physiologic effects, including alteration of platelet function. We aimed to evaluate the effect of hyperlactatemia and subsequent acidemia on platelet aggregation in canine blood using impedance aggregometry. Platelet aggregation was measured in blood from 27 healthy dogs using the Multiplate analyzer at baseline and after in vitro addition of two different volumes of lactic acid to adjust the pH. The area under the curve (AUC), reported by the Multiplate analyzer, was used to assess the extent of platelet aggregation in each sample. A linear mixed effects model was used to test for the association between platelet aggregation and pH. The association of baseline platelet aggregation with HCTs, platelet counts, and WBC counts was assessed using Pearson's correlations. Acidemia was associated with a significant decrease in platelet aggregation. No significant correlations were detected between platelet aggregation and HCT, platelet count, or WBC count. Platelet aggregation measured using the Multiplate analyzer showed substantial individual variation. Worsening acidemia due to the addition of lactic acid caus...Continue Reading

References

Feb 1, 1974·Proceedings of the Society for Experimental Biology and Medicine·E L LamberthR M Des Prez
Apr 1, 1972·Proceedings of the Society for Experimental Biology and Medicine·A B Rogers, R M Des Prez
Sep 7, 2007·Veterinary Clinical Pathology·Connie K StevensonMarion L Jackson
Nov 19, 2009·Annals of Hematology·Benjamin MisselwitzJörg D Seebach
Aug 7, 2012·Journal of Veterinary Internal Medicine·K Hopper, S E Epstein
Sep 29, 2012·Journal of Veterinary Emergency and Critical Care·Clara B MarschnerBo Wiinberg
Mar 4, 2015·Vascular Health and Risk Management·Rita PanicciaRosanna Abbate
Sep 24, 2015·Veterinary Clinical Pathology·Shauna L BloisGabrielle Monteith
Nov 29, 2017·American Journal of Veterinary Research·Katherine J NashElizabeth A Spangler
Feb 28, 2018·Journal of Veterinary Emergency and Critical Care·Andrew G BurtonKate Hopper
Oct 17, 2018·Veterinary Clinical Pathology·Sarah B Shropshire, Christine S Olver

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