The effect of administration of double stranded MicroRNA-210 on acceleration of Achilles tendon healing in a rat model

Journal of Orthopaedic Science : Official Journal of the Japanese Orthopaedic Association
Muhammad Andry UsmanMitsuo Ochi

Abstract

Achilles tendons heal slower than other tissues, therefore requiring the developmnent of a strategy for accelerating the process. Vascular supply plays an important role in primary tendon healing, especially during the early healing phase. MicroRNA (miR)-210 has been reported as being crucial for angiogenesis, which is a key factor of tissue repair. We report herein that local injection of synthetic miR-210 into the injured Achilles tendon of a rat accelerated healing of the tendon. Achilles tendons were transected and repaired via the Kessler suture technique in Sprague-Dawley rats. Then, double stranded (ds) miR-210 was injected into the repaired sites. The control group was injected with non-functioned dsRNA. At 2, 6 and 12 weeks, histological evaluations were performed. At two and six weeks, mechanical testing and angiogenesis were evaluated. Gene expression analysis using real-time polymerase chain reaction (PCR) and immunohistochemistry were performed at two weeks. At two and six weeks, regular dense collagen tissue in the miR-210 group was observed and the diameter of collagen fiber in the miR-210 group was significantly higher than in the control. At two weeks, the ultimate failure load was significantly higher than in ...Continue Reading

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Citations

Jan 3, 2016·Experimental Cell Research·Ying-fei LuJin-fang Zhang
Feb 1, 2014·Foot and Ankle Surgery : Official Journal of the European Society of Foot and Ankle Surgeons·Taner GunesCengiz Sen
Jan 26, 2017·Nature Reviews. Rheumatology·Neal L MillarIain B McInnes
Jun 6, 2020·Annals of the New York Academy of Sciences·Angela Wang IlaltdinovHui B Sun
Jul 5, 2017·Experimental and Therapeutic Medicine·Yong Cao, Pei-Ying Zhang
Dec 28, 2019·International Journal of Molecular Sciences·Germana ZaccagniniFabio Martelli
Mar 29, 2020·British Medical Bulletin·Lorenzo GiordanoNicola Maffulli
Sep 18, 2020·International Journal of Molecular Sciences·Maria Rita CiteroniBarbara Barboni
Jan 1, 2021·Expert Opinion on Biological Therapy·MaCalus V HoganJames H-C Wang
Jan 9, 2021·Nature Reviews. Disease Primers·Neal L MillarScott A Rodeo

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