The effect of age and unilateral leg immobilization for 2 weeks on substrate utilization during moderate-intensity exercise in human skeletal muscle

The Journal of Physiology
Andreas VigelsøJørn W Helge

Abstract

This study aimed to provide molecular insight into the differential effects of age and physical inactivity on the regulation of substrate metabolism during moderate-intensity exercise. Using the arteriovenous balance technique, we studied the effect of immobilization of one leg for 2 weeks on leg substrate utilization in young and older men during two-legged dynamic knee-extensor moderate-intensity exercise, as well as changes in key proteins in muscle metabolism before and after exercise. Age and immobilization did not affect relative carbohydrate and fat utilization during exercise, but the older men had higher uptake of exogenous fatty acids, whereas the young men relied more on endogenous fatty acids during exercise. Using a combined whole-leg and molecular approach, we provide evidence that both age and physical inactivity result in intramuscular lipid accumulation, but this occurs only in part through the same mechanisms. Age and inactivity have been associated with intramuscular triglyceride (IMTG) accumulation. Here, we attempt to disentangle these factors by studying the effect of 2 weeks of unilateral leg immobilization on substrate utilization across the legs during moderate-intensity exercise in young (n = 17; 23 ± ...Continue Reading

References

Dec 1, 1992·Journal of Applied Physiology·D Constantin-TeodosiuE Hultman
Nov 1, 1991·Analytical Biochemistry·D Constantin-TeodosiuE Hultman
Jun 1, 1989·Journal of Applied Physiology·C N MeredithW J Evans
Nov 1, 1993·The American Journal of Physiology·W H MartinJ O Holloszy
Mar 1, 1996·The American Journal of Physiology·L M OdlandL L Spriet
Dec 1, 1996·The American Journal of Physiology·S SialS Klein
Feb 13, 1997·Biochemical and Biophysical Research Communications·B KiensL P Turcotte
Jun 5, 1998·The American Journal of Physiology·L M OdlandL L Spriet
May 19, 2000·The Proceedings of the Nutrition Society·G van HallB Saltin
Mar 13, 2001·International Journal of Obesity and Related Metabolic Disorders : Journal of the International Association for the Study of Obesity·E LevadouxP Ritz
Oct 2, 2001·The Journal of Physiology·L J van LoonA J Wagenmakers
Jan 31, 2002·Annual Review of Medicine·Roger H Unger
Feb 8, 2002·American Journal of Physiology. Endocrinology and Metabolism·T J StephensG K McConell
Oct 16, 2003·American Journal of Physiology. Endocrinology and Metabolism·Margit MahlapuuStefan Marklund
Jan 30, 2004·American Journal of Physiology. Endocrinology and Metabolism·Matthew J WattMark A Febbraio
Mar 23, 2004·Journal of Applied Physiology·Jascha D ParkingtonRoger A Fielding
Aug 5, 2004·The Journal of Clinical Endocrinology and Metabolism·Melanie G CreeRobert R Wolfe
Oct 23, 2004·The Journal of Physiology·Massimo SacchettiGerrit van Hall
Nov 4, 2004·American Journal of Physiology. Endocrinology and Metabolism·Simon SchenkJeffrey F Horowitz
Feb 11, 2005·International Journal of Sports Medicine·A E Jeukendrup, G A Wallis
Oct 14, 2006·The Journal of Physiology·J B Birk, J F P Wojtaszewski
Mar 24, 2007·The Journal of Physiology·Jørn W HelgeBente Kiens
Aug 23, 2007·American Journal of Physiology. Endocrinology and Metabolism·Ditte K KleinJørgen F P Wojtaszewski
Nov 16, 2007·American Journal of Physiology. Endocrinology and Metabolism·Cédric MoroSteven R Smith
Nov 23, 2007·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·B J GurdJ M Kowalchuk
Mar 8, 2008·Journal of Applied Physiology·Micah J DrummondBlake B Rasmussen
Oct 11, 2008·The Journal of Physiology·Nicolas DzamkoGregory R Steinberg
Nov 20, 2008·Diabetes·Audrey BergouignanStéphane Blanc
Dec 25, 2008·American Journal of Physiology. Endocrinology and Metabolism·Thomas J AlstedBente Kiens
Feb 7, 2009·The Biochemical Journal·Erik A Richter, Neil B Ruderman
Feb 25, 2009·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Rémi MounierBenoit Viollet
May 26, 2009·American Journal of Physiology. Heart and Circulatory Physiology·Anthony J DonatoDouglas R Seals
Aug 13, 2009·American Journal of Physiology. Endocrinology and Metabolism·Brynjulf MortensenJørgen F P Wojtaszewski
Dec 10, 2009·American Journal of Physiology. Endocrinology and Metabolism·A C AlibegovicA Vaag
Feb 17, 2010·International Journal of Obesity : Journal of the International Association for the Study of Obesity·M RosenkildeJ W Helge
Nov 12, 2010·The Proceedings of the Nutrition Society·D Grahame Hardie
Jan 18, 2011·Experimental Physiology·Clara PratsAndreas V Hansen
Jan 25, 2011·Scandinavian Journal of Public Health·Louise EriksenTine Curtis

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Citations

Jul 5, 2016·Nutrients·Elfego GalvanDouglas Paddon-Jones
Jul 7, 2016·American Journal of Physiology. Endocrinology and Metabolism·Philip J AthertonCharles H Lang
May 27, 2017·Journal of Applied Physiology·Sune DandanellJørn Wulff Helge
Oct 15, 2019·The Journal of Clinical Endocrinology and Metabolism·Marlou L DirksFrancis B Stephens
May 26, 2016·American Journal of Physiology. Endocrinology and Metabolism·Leslie A ConsittJoseph A Houmard
Jun 4, 2021·European Journal of Trauma and Emergency Surgery : Official Publication of the European Trauma Society·Harry HodgsonAnthony Howard

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