The Effect of Age on the Regenerative Potential of Human Eyelid Adipose-Derived Stem Cells

Stem Cells International
Mingqi ZhangWei He

Abstract

Human eyelid adipose-derived stem cells (HEASCs) are a new source of autologous mesenchymal stem cells, which are derived from neuroectoderm and potentially applied in the tissue regeneration and cell therapies. Based on the prevalence of blepharoplasty in Asia and the availability of HEASCs, we investigated the effect of donor age on their characteristics and regenerative potential of HEASCs in vitro. The HEASCs were isolated from patients of three groups: (1) <20 years (n = 4), (2) >20 years, <45 years (n = 5), and (3) >55 years (n = 4). For each group, the proliferative capacity, colony-forming ability, surface markers, differentiation ability, wound healing function, and secreted protein were contrastively evaluated and quantified for statistical analysis. It was found that HEASCs were successfully isolated and cultured by an explant culture method. The proliferative rates, osteogenic and chondrogenic differentiation potentials, wound healing ability, and the expression of TGF-β1 and fibronectin protein of HEASCs significantly decreased as age increased. However, the expression of CD90 antigen and the adipogenic differentiation showed an age-related increase in HEASCs. As many degenerative diseases increase in prevalence wi...Continue Reading

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Citations

May 18, 2020·Journal of Plastic, Reconstructive & Aesthetic Surgery : JPRAS·Xiyuan MaoYuguang Zhang
Jan 29, 2021·World Journal of Stem Cells·Cintia Ds HorinouchiBruno Dallagiovanna

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Methods Mentioned

BETA
enzyme-linked immunosorbent assay
flow cytometry

Software Mentioned

ImageJ

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