The effect of aging and inflammation on heat shock protein 27 in human monocytes and lymphocytes

Experimental Gerontology
Rose NjeminiTony Mets

Abstract

Heat shock proteins (Hsp) are highly conserved proteins and their synthesis is ubiquitous in virtually every species in which they have been sought. In the present study we have investigated the effect of age and inflammation on the induction of Hsp27 in human peripheral blood mononuclear cells, using flow cytometry. Sixty-six healthy control subjects or patients suffering from inflammation participated in the study. In both heat shocked (HS) and non-HS conditions, the percentage of Hsp27 producing lymphocytes as well as the intensity of Hsp27 in lymphocytes and monocytes were negatively influenced by age. The basal levels and also the levels of Hsp27 production after HS were higher for monocytes compared to lymphocytes. In addition, we found that HS resulted in a small but significant increase in the levels of Hsp27 in lymphocytes whereas a significant decrease in Hsp27 was noticed for monocytes. In conclusion, results presented herein provide evidence in support of an age-related decrease in the level of Hsp27, which disappeared in the presence of inflammation. Several relationships between the circulating levels of CRP, IL-6 and TNF-alpha with the various Hsp27 determinations were observed, indicating that cytokines are able...Continue Reading

Citations

Nov 26, 2010·Cell Stress & Chaperones·Hossein PourghadamyariGordon A Ferns
Mar 11, 2009·Frontiers in Bioscience (Landmark Edition)·Karen L WoodAndrea I Doseff
Jun 18, 2010·Cellular and Molecular Life Sciences : CMLS·Lonneke HeldensNicolette H Lubsen
Jul 20, 2014·Archives of Gynecology and Obstetrics·Li-Qun ZhangJia-Bao Zhang
Jun 14, 2013·Immunity & Ageing : I & a·Karen Henriette PinkeVanessa Soares Lara
Oct 29, 2011·Experimental Gerontology·I BeyerT Mets
May 12, 2010·The Journal of Heart and Lung Transplantation : the Official Publication of the International Society for Heart Transplantation·Karen L WoodAndrea I Doseff
Jul 24, 2007·Mechanisms of Ageing and Development·Rose NjeminiTony Mets
Apr 5, 2007·Journal of Molecular and Cellular Cardiology·Jason N PeartGarretta J Gross
Oct 9, 2009·Acta Clinica Belgica·Ivan BautmansTony Mets
Jun 27, 2008·European Journal of Heart Failure·Alessia MazzolaFranco Laghi-Pasini
Aug 31, 2006·Circulation·Georg Wick
Sep 8, 2007·European Journal of Immunology·Krzysztof LaudanskiCarol Miller-Graziano

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