The effect of alphaB-crystallin and Hsp27 on the availability of translation initiation factors in heat-shocked cells

Cellular and Molecular Life Sciences : CMLS
Linda DoerwaldNicolette H Lubsen

Abstract

The mechanism of the translational thermotolerance provided by the small heat shock proteins (sHsps) alphaB-crystallin or Hsp27 is unknown. We show here that Hsp27, but not alphaB-crystallin, increased the pool of mobile stress granule-associated enhanced green fluorescent protein (EGFP)-eukaryotic translation initiation factor (eIF)4E in heat-shocked cells, as determined by fluorescence recovery after photobleaching. Hsp27 also partially prevented the sharp decrease in the pool of mobile cytoplasmic EGFP-eIF4G. sHsps did not prevent the phosphorylation of eIF2alpha by a heat shock, but promoted dephosphorylation during recovery. Expression of the C-terminal fragment of GADD34, which causes constitutive dephosphorylation of eIF2alpha, fully compensated for the stimulatory effect of alphaB-crystallin on protein synthesis in heat-shocked cells, but only partially for that of Hsp27. Our data show that sHsps do not prevent the inhibition of protein synthesis upon heat shock, but restore translation more rapidly by promoting the dephosphorylation of eIF2alpha and, in the case of Hsp27, the availability of eIF4E and eIF4G.

Citations

Dec 15, 2007·American Journal of Respiratory Cell and Molecular Biology·Steven G KelsenSalim Merali
Aug 20, 2011·PloS One·Lonneke HeldensNicolette H Lubsen
Jun 18, 2010·Cellular and Molecular Life Sciences : CMLS·Lonneke HeldensNicolette H Lubsen
Sep 20, 2006·Expert Opinion on Therapeutic Targets·Hong Pyo KimAugustine M K Choi
Nov 25, 2006·Experimental Cell Research·A L BryantsevE A Shelden
Nov 23, 2006·Biochimica Et Biophysica Acta·Laura Marín-VinaderNicolette H Lubsen
Mar 13, 2010·Biopolymers·Jens BuchardtNils Langeland Johansen
Jan 1, 2013·Computational and Structural Biotechnology Journal·Malek KammounIsabelle Cassar-Malek
Dec 29, 2009·Biochimica Et Biophysica Acta·Elena S NadezhdinaPavel A Ivanov
Oct 8, 2016·Journal of Molecular Histology·Gali Epstein ShochetShelly Tartakover Matalon

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