The effect of amino acid infusion on leg protein turnover assessed by L-[15N]phenylalanine and L-[1-13C]leucine exchange

European Journal of Clinical Investigation
W M BennetMichael J Rennie

Abstract

A stable isotope technique depending on the use of [15N]phenylalanine and [1-13C]leucine to assess exchange was utilized to measure the components of protein turnover of the human leg and the effects of amino acid infusion. Eight healthy subjects (28.5 +/- 2.5 years) were studied when post-absorptive in the basal state and again during infusion of a mixed amino acid solution (55 g l-1, 1.52 ml kg-1 h-1). During the basal period leucine oxidation by the leg was 4.4 +/- 2.0 nmol 100 g-1 min-1 and this increased threefold during amino acid infusion (13.6 +/- 3.1 nmol 100 g-1 min-1, mean +/- SEM, P = 0.003). Amino acid infusion abolished the net negative balance between incorporation of leucine into, and release from, protein (basal, -31.8 +/- 5.8; during infusion, +3.1 +/- 7.1 nmol 100 g-1 P = 0.001). Phenylalanine exchange showed a similar pattern (basal, -13.7 +/- 1.8; during infusion, -0.8 +/- 3.0 nmol 100 g-1 min-1, P = 0.003). Basal entry of leucine into leg protein (i.e. protein synthesis) was 70.0 +/- 10.8 nmol 100 g-1 min-1 and this increased during amino acid infusion to 87.3 +/- 14.1 nmol 100 g-1 min-1 (P = 0.11). Phenylalanine entry to protein also increased with amino acid infusion (29.1 +/- 4.5 vs. 38.3 +/- 5.8 nmol 1...Continue Reading

References

Jan 1, 1985·The International Journal of Biochemistry·E M Tyobeka, K L Manchester
Feb 15, 1986·British Medical Journal·P J IllingworthT E Isles
Feb 1, 1988·Clinical Science·K J TraceyS F Lowry
Jul 1, 1987·The Journal of Clinical Investigation·R A Gelfand, E J Barrett
Sep 1, 1987·The Journal of Clinical Investigation·K LundholmA C Möller-Loswick
Nov 1, 1987·Journal of Neurology, Neurosurgery, and Psychiatry·P DietrichsonR H Edwards
Jan 1, 1968·Metabolism: Clinical and Experimental·B IssekutzW M Bortz
Dec 4, 1981·Science·D E MatthewsG A Clugston

❮ Previous
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Citations

Apr 1, 1991·Diabetic Medicine : a Journal of the British Diabetic Association·W M Bennet, M J Rennie
Jan 21, 2000·Current Opinion in Clinical Nutrition and Metabolic Care·P Tessari, G Garibotto
Jan 22, 2003·Scandinavian Journal of Medicine & Science in Sports·J Dorrens, M J Rennie
Oct 22, 2002·American Journal of Physiology. Endocrinology and Metabolism·Pamela M J O'ConnorTeresa A Davis
May 12, 2005·American Journal of Physiology. Endocrinology and Metabolism·Thomas C VaryChristopher J Lynch
Jun 26, 2008·American Journal of Physiology. Endocrinology and Metabolism·P L GreenhaffM J Rennie
Jul 11, 2008·The Journal of Nutrition, Health & Aging·Y RollandB Vellas
Oct 9, 2009·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Hélène FouilletCécile Bos
Aug 15, 2014·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Anthony M J SanchezRobin B Candau
Feb 9, 2000·American Journal of Physiology. Endocrinology and Metabolism·M Sheffield-MooreA A Ferrando
Apr 8, 1998·The American Journal of Physiology·X J ZhangR R Wolfe
Jun 12, 2004·Current Opinion in Clinical Nutrition and Metabolic Care·Elena VolpiSatoshi Fujita
Apr 9, 2008·Annual Review of Nutrition·Satish C Kalhan, Dennis M Bier
Dec 5, 2006·Current Opinion in Clinical Nutrition and Metabolic Care·Satish C Kalhan, John M Edmison
Sep 6, 2007·Journal of Animal Science·T A DavisM L Fiorotto
Jun 1, 2004·Nutrition Research Reviews·Thomas C Vary, Christopher J Lynch
May 3, 2007·Medicine and Science in Sports and Exercise·Patricia Courtney GaineNancy R Rodriguez
Nov 25, 2005·Pediatric Research·Prabhu S ParimiSatish C Kalhan
Feb 24, 2004·Annual Review of Physiology·Michael J RennieFrank W Booth
Jan 1, 2008·Nutrition Today·Teresa A Davis, UNKNOWN The 2007 Avanelle Kirksey Lecture, Purdue University
Apr 28, 2016·Applied Physiology, Nutrition, and Metabolism = Physiologie Appliquée, Nutrition Et Métabolisme·Bethan E PhillipsKenneth Smith
Apr 18, 2015·Critical Care : the Official Journal of the Critical Care Forum·Felix LiebauOlav Rooyackers
Jan 1, 1995·JPEN. Journal of Parenteral and Enteral Nutrition·A A FerrandoR R Wolfe
Apr 1, 1994·The American Journal of Physiology·A C Möller-LoswickK Lundholm
Oct 1, 1995·Intensive Care Medicine·G IapichinoM Savioli
Aug 15, 2000·Annual Review of Nutrition·M J Rennie, K D Tipton
Mar 8, 2002·American Journal of Physiology. Endocrinology and Metabolism·Teresa A DavisPamela M J O'Connor
Oct 29, 2000·American Journal of Physiology. Endocrinology and Metabolism·P G WhittakerR Taylor
Nov 3, 2005·American Journal of Physiology. Endocrinology and Metabolism·Mark M KadrofskeSatish C Kalhan
Jul 20, 2007·The Journal of Nutrition·Thomas C Vary, Christopher J Lynch
Feb 1, 1992·The American Journal of Physiology·S InchiostroP Tessari
Mar 1, 1994·The American Journal of Physiology·M J RennieP W Watt
Aug 1, 1990·The American Journal of Physiology·W M BennetM J Rennie
Jan 1, 1995·The American Journal of Physiology·A E el-KhouryV R Young
Jan 1, 2010·Animal : an International Journal of Animal Bioscience·T A DavisD G Burrin

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