The effect of aminosulfonate buffers on the light responses and intracellular pH of goldfish retinal horizontal cells

Journal of Neurochemistry
Stuart Trenholm, William H Baldridge

Abstract

Retinal horizontal cell feedback acts as a gain control at the first synapse in the visual system and generates center-surround receptive fields in the outer retina. One model of feedback proposes that elevation of protons in the photoreceptor synaptic cleft produces feedback. Most evidence supporting the proton model has depended on the effect of proton buffers, in particular aminosulfonates, but these agents could potentially have effects other than external pH regulation. We therefore determined if the effects of aminosulfonates on horizontal cell rollback, an indicator of feedback, were consistent with external proton buffering. Intracellular recording from horizontal cells in isolated goldfish retina revealed that rollback was blocked only by aminosulfonates with an acid dissociation constant suited for buffering at the pH (7.5) of the Ringer's solution. In isolated goldfish horizontal cells, aminosulfonates, even those that did not block rollback, altered intracellular pH. This suggests that the effect of aminosulfonates on rollback is not because of changing intracellular pH. Measures of both intracellular and extracellular pH revealed that treatment with either glutamate or kainate resulted in acidification. As glutamat...Continue Reading

References

Oct 1, 1989·The Journal of General Physiology·S Barnes, B Hille
Nov 1, 1986·Biophysical Journal·N A Curtin
Apr 1, 1971·The Journal of Physiology·D A BaylorP M O'Bryan
Dec 30, 1999·The Journal of General Physiology·D A KraaijM Kamermans
May 12, 2001·Science·M KamermansR Weiler
Oct 21, 2003·The Journal of Comparative Neurology·Mark PottekReto Weiler
Jul 24, 2004·The Journal of Physiology·Anthony J A MolinaRobert Paul Malchow
Apr 22, 2005·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·John P VesseySteven Barnes
Jan 18, 2007·The Journal of Biological Chemistry·Jinshu YuGina E Sosinsky
Feb 15, 2007·The Journal of Comparative Neurology·Colleen R ShieldsMaarten Kamermans
Mar 3, 2007·The Journal of Physiology·Michael G Jonz, Steven Barnes
Aug 1, 2007·The Journal of General Physiology·Matthew A KreitzerRobert Paul Malchow
Jan 11, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Christopher M DavenportDennis M Dacey
May 30, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Wallace B ThoresonTheodore M Bartoletti

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Citations

May 20, 2011·Journal of Neurophysiology·Emily McMains, Evanna Gleason
Jan 21, 2014·Nature Neuroscience·Tzu-Ming WangRichard H Kramer
May 15, 2012·Progress in Retinal and Eye Research·Wallace B Thoreson, Stuart C Mangel
Dec 15, 2015·PLoS Biology·Richard H Kramer, Christopher M Davenport
May 30, 2019·Physiological Reviews·Wallace B Thoreson, Dennis M Dacey
Jun 16, 2017·Annual Review of Vision Science·Jeffrey S Diamond

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