Abstract
Addition of ammonium chloride to interferon (IFN)-treated mouse L cells has been reported to reduce the antiviral state. Because (2'-5')-(A)n synthetase and (2'-5')-(A)n-dependent endoribonuclease (RNase L) activities have been implicated in the establishment of the antiviral state, we wish to ascertain if ammonium ions will also impair the IFN-elicited induction of these two enzymatic activities. To test this possibility, cell-free extracts were prepared from mouse BALB/C 3T3 cells and assayed for (2'-5')-(A)n synthetase and RNase L activities. Results of these studies show that when cells were incubated with IFN (100 or 500 U/ml) and ammonium chloride (20-40 mM) for 20 h, the induction of (2'-5')-(A)n synthetase by IFN is significantly suppressed. Based on binding to the specific radioactive (2'-5')-(A)n analog, (2'-5')p3A4,3'-[32P]pCp, on cross-linking to the periodate-oxidized (2'-5')p3A4,3'-[32P]pC, or on the (2'-5')-(A)n-enhanced degradation of [3H]-polyadenylated RNA, IFN (10-250 U/ml) was shown to cause a two- to sevenfold increase in RNase L activity. The induction of RNase L by IFN was blocked by simultaneous addition of ammonium chloride. However, 20 mM or 40 mM ammonium chloride did not affect the antiviral state in...Continue Reading
References
Dec 6, 1979·Nature·B R WilliamsI M Kerr
Dec 1, 1978·The Journal of General Virology·M J Commoy-ChevalierC Chany
May 1, 1979·Biochemistry·C BaglioniD K West
Jun 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·T W Nilsen, C Baglioni
Sep 1, 1979·FEBS Letters·B R WilliamsI M Kerr
Jul 31, 1985·Biochemical and Biophysical Research Communications·J M WuB Eslami
May 1, 1980·Journal of Virology·B Y Rubin, S L Gupta
Apr 10, 1981·Nucleic Acids Research·D H WreschnerI M Kerr
Mar 1, 1982·Virology·P AndersonJ Vilcek
May 1, 1982·European Journal of Biochemistry·R H SilvermanI M Kerr
Jan 1, 1982·Annual Review of Biochemistry·P Lengyel
Feb 1, 1980·Virology·A G Hovanessian, J N Wood
May 17, 1982·European Journal of Biochemistry·D H WreschnerI M Kerr
Feb 1, 1981·Journal of Interferon Research·A G HovanessianL Montagnier
Oct 1, 1984·Cell·R L FriedmanG R Stark
Oct 18, 1983·Biochimica Et Biophysica Acta·T J BorelliJ M Wu
Nov 30, 1983·Biochemical and Biophysical Research Communications·J M WuA R Semproni
Feb 14, 1984·Biochimica Et Biophysica Acta·A R Semproni, J M Wu
Mar 16, 1981·European Journal of Biochemistry·R H SilvermanI M Kerr