The effect of ammonium ion on the induction of (2'-5')-(A)n synthetase and (2'-5')-(A)n-dependent endoribonuclease activities by interferon in mouse BALB/C 3T3 cells

Journal of Interferon Research
J M WuR Alessandroni

Abstract

Addition of ammonium chloride to interferon (IFN)-treated mouse L cells has been reported to reduce the antiviral state. Because (2'-5')-(A)n synthetase and (2'-5')-(A)n-dependent endoribonuclease (RNase L) activities have been implicated in the establishment of the antiviral state, we wish to ascertain if ammonium ions will also impair the IFN-elicited induction of these two enzymatic activities. To test this possibility, cell-free extracts were prepared from mouse BALB/C 3T3 cells and assayed for (2'-5')-(A)n synthetase and RNase L activities. Results of these studies show that when cells were incubated with IFN (100 or 500 U/ml) and ammonium chloride (20-40 mM) for 20 h, the induction of (2'-5')-(A)n synthetase by IFN is significantly suppressed. Based on binding to the specific radioactive (2'-5')-(A)n analog, (2'-5')p3A4,3'-[32P]pCp, on cross-linking to the periodate-oxidized (2'-5')p3A4,3'-[32P]pC, or on the (2'-5')-(A)n-enhanced degradation of [3H]-polyadenylated RNA, IFN (10-250 U/ml) was shown to cause a two- to sevenfold increase in RNase L activity. The induction of RNase L by IFN was blocked by simultaneous addition of ammonium chloride. However, 20 mM or 40 mM ammonium chloride did not affect the antiviral state in...Continue Reading

References

Dec 1, 1978·The Journal of General Virology·M J Commoy-ChevalierC Chany
Jun 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·T W Nilsen, C Baglioni
Sep 1, 1979·FEBS Letters·B R WilliamsI M Kerr
Mar 1, 1982·Virology·P AndersonJ Vilcek
May 1, 1982·European Journal of Biochemistry·R H SilvermanI M Kerr
Jan 1, 1982·Annual Review of Biochemistry·P Lengyel
Feb 1, 1980·Virology·A G Hovanessian, J N Wood
Oct 18, 1983·Biochimica Et Biophysica Acta·T J BorelliJ M Wu
Nov 30, 1983·Biochemical and Biophysical Research Communications·J M WuA R Semproni
Feb 14, 1984·Biochimica Et Biophysica Acta·A R Semproni, J M Wu

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