PMID: 2493385Jan 1, 1989Paper

The effect of an aldose reductase inhibitor on lens phosphorylcholine under hyperglycemic conditions: biochemical and NMR studies

Experimental Eye Research
M F LouH M Jernigan

Abstract

Phosphorylcholine (P-choline) is a precursor of the phospholipids in the lens membrane. A human lens normally contains approx. 1 mM P-choline but this is significantly lowered in some cataractous lenses. A normal rat lens contains a very high concentration (11 mM). We found that rat lens P-choline was depleted drastically when the lenses were exposed to hyperglycemic conditions either in culture, with galactose or xylose, or in vivo by streptozotocin-induced diabetes. The lens P-choline level was measured by fractionating the organic phosphates in the lens homogenate using an ion exchange column, or by quantitating the P-choline 31P NMR intensity in intact lenses. The results of both the chemical method and the noninvasive method agreed remarkably well. Besides the change in P-choline, the choline influx was also drastically reduced both in lenses from diabetic rats and in lenses incubated with 30 mM xylose. In addition, the ATP concentration was greatly diminished under similar conditions. The changes in P-choline, choline, and ATP could all be prevented in the presence of an aldose reductase inhibitor (ARI). It is thus concluded that these changes in phospholipid precursors may result from lenticular membrane defects caused b...Continue Reading

References

May 28, 1979·Biochemical and Biophysical Research Communications·P J Geiger, C M Roberts
Apr 1, 1988·Experimental Eye Research·M F LouB M York
Nov 1, 1984·Current Eye Research·P S Zelenka
Feb 1, 1982·Experimental Eye Research·P S Zelenka, H M Jernigan
Jun 1, 1981·Experimental Eye Research·H M JerniganJ H Kinoshita
Jan 1, 1981·Current Eye Research·S J KoppT Glonek

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Citations

May 1, 1995·NMR in Biomedicine·J C WatertonF Carey
Apr 22, 2010·Journal of Lipid Research·Douglas Borchman, Marta C Yappert
Mar 29, 2003·Diabetes·D Steven ZatechkaMarjorie F Lou
Dec 20, 2005·Experimental Eye Research·Jhang Ho PakHungwon Tchah
Feb 1, 1995·Current Eye Research·J E DickersonR W Gracy
Nov 1, 1989·Experimental Eye Research·H M Jernigan, J S Zigler

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