The effect of an RGD-containing fusion protein CBD-RGD in promoting cellular adhesion

Journal of Biotechnology
Shan-hui HsuChing-Lin Tsai

Abstract

The effect of a recombinant RGD (arginine-glycine-aspartic acid)-containing fusion protein, cellulose-binding domain (CBD)-RGD, on the cellular adhesion to a biomedical polyurethane (PU) was evaluated. A series of different cell lines, as well as freshly harvested animal cells, were grown on the PU surfaces with or without CBD-RGD, in serum or serum-free media. The results showed that the enhancement of cellular attachment by CBD-RGD varied with cell types. This is believed to be a result of the unique integrin receptors on each type of cell surface. The existence of certain divalent ions (Mg2+ and Mn2+) may increase the efficacy of the CBD-RGD, in a cell type-dependent manner. The fusion protein was also found to inhibit the platelet activation. The effect of CBD-RGD was further examined in two other substrate materials, poly(L-lactide) (PLLA) and poly(lactide-co-glycolide) (PLGA). The effect on cellular adhesion correlated with the amount of CBD-RGD physically adsorbed on the material surface.

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Citations

Jan 1, 2009·Biotechnology Letters·Robert E NordonFrances C Foong
Nov 28, 2009·Journal of Materials Science. Materials in Medicine·Jürgen Rudolf Josef PalettaMarkus Dietmar Schofer
Jun 9, 2006·Microbiology and Molecular Biology Reviews : MMBR·Oded ShoseyovIlan Levy
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Jul 1, 2008·Journal of Biotechnology·Ryou-u TakahashiHiroshi Handa
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Oct 4, 2008·Biomacromolecules·Achala de MelAlexander M Seifalian

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