The effect of angiotensin II on intracellular pH is mediated by AT(1) receptor translocation

American Journal of Physiology. Cell Physiology
Karina ThiemeMaria Oliveira-Souza

Abstract

The effect of ANG II on intracellular pH (pH(i)) recovery rate and AT(1) receptor translocation was investigated in transfected MDCK cells. The pH(i) recovery rate was evaluated by fluorescence microscopy using the fluorescent probe BCECF-AM. The human angiotensin II receptor isoform 1 (hAT(1)) translocation was analyzed by immunofluorescence and confocal microscope. Our data show that transfected cells in control situation have a pH(i) recovery rate of 0.219 +/- 0.017 pH U/min (n = 11). This value was similar to nontransfected cells [0.211 +/- 0.009 pH U/min (n = 12)]. Both values were significantly increased with ANG II (10(-9) M) but not with ANG II (10(-6) M). Losartan (10(-7) M) and dimethyl-BAPTA-AM (10(-7) M) decreased significantly the stimulatory effect of ANG II (10(-9) M) and induced an increase in Na(+)/H(+) exchanger 1 (NHE-1) activity with ANG II (10(-6) M). Immunofluorescence studies indicated that in control situation, the hAT(1) receptor was predominantly expressed in cytosol. However, it was translocated to plasma membrane with ANG II (10(-9) M) and internalized with ANG II (10(-6) M). Losartan (10(-7) M) induced hAT(1) translocation to plasma membrane in all studied groups. Dimethyl-BAPTA-AM (10(-7) M) did no...Continue Reading

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Citations

Nov 10, 2012·Journal of Receptor and Signal Transduction Research·Chung-Pin LiuChung-Ren Jan
Sep 26, 2013·Journal of Receptor and Signal Transduction Research·Chung-Pin LiuChung-Ren Jan
Mar 11, 2016·American Journal of Physiology. Renal Physiology·Fernando Augusto Malavazzi CasareMaria Oliveira-Souza

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