PMID: 1195342Apr 23, 1975Paper

The effect of anti-L on ouabain binding to sheep erythrocytes

The Journal of Membrane Biology
C H Joiner, P K Lauf

Abstract

Binding of 3H-ouabain was studied in high potassium (HK) and low potassium (LK) sheep red cells. In particular, we investigated the effect of anti-L, an antibody raised in HK sheep against L-positive LK sheep red cells, on 3H-oubain binding and its relation to K+ -pump flux inhibition in LK cells. HK cells were found to have about twice as many 3H-ouabain binding sites and a higher association rate for 3H-ouabain than homozygous LL-type LK cells. The number of 3H-ouabain molecules bound to heterozygous LM-type LK cells is lower than that on LL cells, but the rate of ouabain binding is between that of HK and LL red cells. A close correlation was observed between the rates of 3H-oubain binding and fraction K+-pump inhibition. Exposure of LM and LL cells to anti-L did not affect the number of 3H-ouabain molecules bound at saturation, but increased the rates of glycoside binding and K+ -pump inhibition proportionately, so that for LK cells in the presence of anti-L, the rates of the two processes approximate those of HK cells. These data exclude the possibility that anti-L generates entirely new pump sites in LK sheep cells, but suggest that the antibody increases the affinity of the existing -a+ -K+ pumps for the glycoside.

References

Jan 1, 1970·Biochimica Et Biophysica Acta·J C Ellory, E M Tucker
Mar 1, 1972·The Journal of General Physiology·Y IsraelE Rosenmann
Jul 1, 1972·The Journal of Physiology·P F Baker, J S Willis
Jan 1, 1974·Annals of the New York Academy of Sciences·P K Lauf
Nov 1, 1972·The Journal of General Physiology·J D Gardner, T P Conlon
Apr 1, 1974·The Journal of General Physiology·J R SachsJ F Hoffman
Jan 1, 1974·The Journal of Membrane Biology·J D Gardner, C Frantz
May 9, 1972·Biochimica Et Biophysica Acta·G R Kepner, D C Tosteson
Oct 1, 1971·The Journal of General Physiology·P G Hoffman, D C Tosteson
Nov 1, 1966·The American Journal of Medicine·J F Hoffman
May 1, 1971·The Journal of General Physiology·L A Beauge, N Adragna
Jul 1, 1971·The Journal of General Physiology·P B Dunham, J F Hoffman
Nov 14, 1957·The Journal of Physiology·J V EVANS, A T PHILLIPSON
Sep 1, 1960·The Journal of General Physiology·D C TOSTESON, J F HOFFMAN
Dec 1, 1969·The Journal of Membrane Biology·P K Lauf, D C Tosteson
Dec 1, 1970·The Journal of Membrane Biology·P K LaufD C Tosteson

❮ Previous
Next ❯

Citations

Sep 17, 1976·The Journal of Membrane Biology·P K Lauf, W W Sun
Dec 14, 1976·Biochimica Et Biophysica Acta·P B Dunham, R Blostein
Jul 1, 1982·Clinica Chimica Acta; International Journal of Clinical Chemistry·K Narayanareddy, S S Kaplay
Jul 1, 1980·The Journal of General Physiology·H D KimP K Lauf
Mar 1, 1983·The Journal of General Physiology·D G Shoemaker, P K Lauf
Aug 1, 1981·The Journal of Clinical Investigation·D M KajiT Kahn
Apr 19, 1979·Biochimica Et Biophysica Acta·C H Joiner, P K Lauf
Jul 1, 1983·Metabolism: Clinical and Experimental·K Narayanareddy, S S Kaplay
Oct 15, 1976·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·E M TuckerJ Herbert
Jan 1, 1976·Animal Blood Groups and Biochemical Genetics·E M Tucker
Mar 28, 1986·Biochemical and Biophysical Research Communications·P OttolenghiJ Jensen

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cardiac Glycosides

Cardiac glycosides are a diverse family of naturally derived compounds that bind to and inhibit na+/k+-atpase. Discover the latest research on cardiac glycosides heres.