PMID: 6989829May 10, 1980Paper

The effect of antibodies against Escherichia coli small ribosomal subunit proteins on protein synthesis by rat liver ribosomes.

The Journal of Biological Chemistry
T TanakaG Stöffler

Abstract

Monovalent Fab fragments from immunoglobulins, directed against Escherichia coli small ribosomal subunit proteins, were tested for their effect on poly(U)-directed synthesis of polyphenylalanine by rat liver ribosomes. Of the 18 Fabs tested, 3, anti-S10, anti-S12, and anti-S14, inhibited polyphenylalanine synthesis; antibodies against 3 of the 21 E. coli ribosomal proteins (S1, S16, and S17) were not tested. The others were either without any appreciable effect or, as in the case of anti-S11 and anti-S18, inhibited far less. Antibodies against S12 and S14 (but not against S10) inhibited elongation factor 1-dependent binding of [14C]Phe-tRNA to rat liver 80 S ribosomes. The presumption is that the prokaryotic (E. coli) ribosomal proteins, S10, S12, and S14, possess antigenic determinants also present in eukaryotic (rat liver) ribosomal proteins; the rat liver proteins have not yet been identified.

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