The effect of arachidonic acid and free fatty acids on vesicular uptake of glutamate and gamma-aminobutyric acid

European Journal of Pharmacology
S RosethF Fonnum

Abstract

The manner in which arachidonic acid and other free fatty acids influence the vesicular uptake of glutamate and gamma-aminobutyric acid (GABA) has been investigated. The cis-polyunsaturated fatty acid arachidonic acid (20:4), eicosapentanoic acid (20:5) and linolenic acid (18:3) at 150 nmol/mg protein (50 microM) inhibited the vesicular uptake of glutamate and GABA more than 70%. Reduced inhibition of vesicular uptake was seen with the cis-monounsaturated fatty acid oleic acid (18:1) and the trans-mono-unsaturated fatty acid elaidic acid (18:1). The saturated fatty acids stearic acid (16:0) and arachidic acid (20:0) had no significant effect on the uptake. The inhibition of vesicular uptake by arachidonic acid was prevented by the addition of fatty acid free bovine serum albumin. Arachidonic acid inhibited in a dose-dependent manner the generation of the transmembrane pH gradient of the synaptic vesicles. This inhibition was proportional to the inhibition of the vesicular uptake of glutamate and GABA. The saturated fatty acid arachidic acid showed no inhibition of delta pH generation. Arachidonic acid at 200 nmol/mg of protein did not increase the uptake-independent leakage of glutamate and GABA from the vesicles, showing that ...Continue Reading

References

Jan 1, 1992·Advances in Experimental Medicine and Biology·J W LazarewiczJ T Wroblewski
Mar 1, 1990·Trends in Neurosciences·P R MaycoxR Jahn
Mar 11, 1991·Trends in Neurosciences·R W OrdwayJ V Walsh
Oct 1, 1990·The Biochemical Journal·F ZafraC Gimenez
May 15, 1989·Biochemical and Biophysical Research Communications·O HolianL M Nyhus
May 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·P E KishT Ueda
Jan 1, 1985·Journal of Neurochemistry·S Naito, T Ueda
Jul 1, 1985·Journal of Neurochemistry·H YasudaM Nakanishi
Oct 10, 1970·Nature·K Krnjević
Oct 15, 1982·Biochemical and Biophysical Research Communications·J K DisbrowJ A Ruth
Jan 1, 1984·Journal of Neurochemistry·F Fonnum
May 1, 1982·Journal of Neurochemistry·D E RhoadsE Raghupathy
Apr 1, 1981·Journal of Neurochemistry·M GardinerB K Siesjö
May 1, 1980·Acta Physiologica Scandinavica·C D AgardhB K Siesjö
Mar 24, 1995·The Journal of Biological Chemistry·N ZerangueM P Kavanaugh

❮ Previous
Next ❯

Citations

Feb 10, 2004·Progress in Retinal and Eye Research·Neville N OsborneJosé Melena
Feb 16, 2000·Neuroreport·R G TavaresC S Dutra-Filho
Sep 22, 2007·Annual Review of Pharmacology and Toxicology·Farrukh A ChaudhryFrode Fonnum
Jan 11, 2020·Biochimica Et Biophysica Acta. Biomembranes·Charles M Thompson, Chih-Kai Chao
May 16, 2021·Neuropharmacology·Ada G Rodríguez-Campuzano, Arturo Ortega

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.