The effect of assay methods on plasma levels and pharmacokinetics of theophylline: HPLC and EIA

British Journal of Clinical Pharmacology
T IshizakiN Morishita

Abstract

1 The effects of two assay methods, HPLC and EIA, on theophylline plasma levels (n = 254) and pharmacokinetics (n = 6) were examined on blood samples obtained from asthmatic patients and normal adult male volunteers. 2 The standard curves obtained and the plasma level values during plasma level monitoring of theophylline measured with two methods are well correlated each other within concentration range of 2.5 to 20.0 microgram/ml (P less than 0.001). EIA, however in general, appears to give a lower value than HPLC. 3 The values of plasma theophylline half-life and clearance analyzed using a two-compartment open system model were found different between the two assay methods and dissimilar to those previously reported by using ultraviolet method. 4 The sampling end-point and/or assay sensitivity particularly to measure a lower level of theophylline (less than 2.5 microgram/ml) can influence the pharmacokinetics for a potent bronchodilator with a narrow therapeutic margin, theophylline.

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Citations

May 7, 2002·Journal of Controlled Release : Official Journal of the Controlled Release Society·Mariko MorishitaNicholas A Peppas
Jan 1, 1988·The Journal of International Medical Research·D Bernocchi, C L Castiglioni
Nov 1, 1987·The Journal of International Medical Research·S GiosueP Mancini
Oct 1, 1981·Journal of Pharmacokinetics and Biopharmaceutics·P ChennavasinD C Brater

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