PMID: 9160410Aug 1, 1996Paper

The effect of bacterial toxins on levels of intracellular adenosine nucleotides and human ciliary beat frequency

Pulmonary Pharmacology
K KanthakumarR Wilson

Abstract

Toxins that slow ciliary beat are virulence determinants of bacteria that infect or invade ciliated epithelial surfaces. We have previously shown that the effect of the Pseudomonas aeruginosa toxin pyocyanin on ciliary beat is associated with a fall in intracellular cAMP and ATP. We have now investigated whether reduction in intracellular adenosine nucleotides might be a common mechanism of action of other bacterial toxins which slow ciliary beat. Two other P. aeruginosa toxins, 1-hydroxyphenazine (1-HP) and rhamnolipid, and two Haemophilus influenzae fractions produced by gel filtration of broth cultures were tested. The effect on human nasal epithelium ciliary beat frequency (CBF), and intracellular cAMP and ATP were measured, and the effect of two pharmacological agents, dibutyryl cAMP and salmeterol, on these changes was assessed. 1-HP, rhamnolipid and the two H. influenzae fractions slowed CBF before there was significant release of lactate dehydrogenase from the cells. The toxins also caused a fall in intracellular cAMP and ATP. Dibutyryl cAMP and salmeterol at the concentrations used do not increase baseline CBF, but diminished the fall in CBF and intracellular adenosine nucleotides. The cAMP and ATP levels in these stud...Continue Reading

Citations

Aug 9, 2011·American Journal of Rhinology & Allergy·Ke-Qing ZhaoNoam A Cohen
Nov 16, 2016·Cells·Asma Yaghi, Myrna B Dolovich
Dec 31, 2019·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Janna C NawrothEva Kanso
Nov 5, 2002·American Journal of Physiology. Lung Cellular and Molecular Physiology·Yunxia Q O'MalleyBradley E Britigan
Mar 29, 2002·Pediatric Pulmonology·Michael Robinson, Peter T B Bye
Mar 26, 2010·Applied Microbiology and Biotechnology·Ahmad Mohammad Abdel-MawgoudEric Déziel
Dec 17, 2014·American Journal of Rhinology & Allergy·Alan D Workman, Noam A Cohen
Sep 15, 2005·Applied Microbiology and Biotechnology·Gloria Soberón-ChávezEric Déziel
Apr 29, 1998·Antimicrobial Agents and Chemotherapy·A RutmanR Wilson
Dec 5, 2002·The Journal of Allergy and Clinical Immunology·Matthias Salathe
Jun 29, 2021·Virulence·Charles D MorinGee W Lau
Jun 14, 2005·Trends in Microbiology·Gee W LauBradley E Britigan

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