PMID: 6111345Mar 6, 1981Paper

The effect of bilayer thickness on the activity of (Na+ + K+)-ATPase

Biochimica Et Biophysica Acta
A JohannssonJ C Metcalfe

Abstract

The activities of purified (Na+ + K+)-ATPase supported by a series of phosphatidylcholines with monounsaturated (cis-9) fatty acyl chains (di(n : 1) phosphatidylcholine) varying in length from n = 12 to n = 23 were determined by the lipid titration technique. The ATPase activity at 20 degrees C decreased from 2.9 to 0.1 mumol/min per mg protein as n was decreased from 16 to 12 and decreased from 2.9 to 1.0 mumol/min per mg protein as n was increased from 20 to 23. In further experiments, the di(n : 1) phosphatidylcholine-ATPase complexes were treated with increasing proportions of n-decane, which has been shown previously to increase the thickness of black lipid membranes. n-Decane caused a large increase (greater than 20-fold) in activity of the short-chain complexes (n = 12,13); for n = 14--18, the ATPase activity first increased and subsequently decreased as the proportion of decane was increased, and for n = 20 or 23 decane caused a progressive decrease in activity with increasing concentration. These effects confirm qualitatively that a major factor determining the activity in each bilayer is its thickness. This behaviour closely parallels that of the (Ca2+ + Mg2+)-ATPase of sarcoplasmic reticulum [1] and suggests that a m...Continue Reading

References

Jul 28, 1977·Nature·D A HaydonJ Requena
Sep 1, 1971·The Journal of Membrane Biology·R FettiplaceD A Haydon

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Citations

Jan 1, 1993·European Biophysics Journal : EBJ·M M Sperotto, O G Mouritsen
Dec 19, 2009·European Biophysics Journal : EBJ·Andrea Holt, J Antoinette Killian
Dec 15, 1995·Clinica Chimica Acta; International Journal of Clinical Chemistry·J F Dwight, B M Hendry
Jan 1, 1986·The International Journal of Biochemistry·E Zsigmond, M T Clandinin
Apr 1, 1994·Progress in Neurobiology·J R Elliott, A A Elliott
Feb 1, 1990·The Journal of Nutritional Biochemistry·M G Murphy
Nov 7, 1998·Biochimica Et Biophysica Acta·J A Killian
May 11, 2011·Nature Communications·Yonathan SonntagLea Thøgersen
Mar 23, 2000·European Journal of Biochemistry·S SennouneJ M Maixent
Mar 16, 2004·Proceedings of the National Academy of Sciences of the United States of America·Kakoli MitraDonald M Engelman
Jan 1, 1987·Biotechnology & Genetic Engineering Reviews·S L Neidleman
Dec 1, 1992·Photochemistry and Photobiology·Z T FarahbakhshW L Hubbell
Oct 16, 2009·Molecular Interventions·Christopher B Divito, Susan G Amara
Feb 1, 2007·Annual Review of Biophysics and Biomolecular Structure·Olaf S Andersen, Roger E Koeppe
Apr 15, 2011·Journal of Lipids·Katie D Hickey, Mary M Buhr
Oct 26, 2001·Hypertension Research : Official Journal of the Japanese Society of Hypertension·T MiyajimaM Yokoyama
Sep 16, 2015·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Bożena BukowskaMaria Koter-Michalak
May 1, 1983·Archives of Biochemistry and Biophysics·J Y Westcott, H Weiner
Aug 6, 2015·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Maria João LançaPedro Raposo de Almeida
May 17, 2012·Biochimica Et Biophysica Acta·A Srinivas ReddyMirianas Chachisvilis
Mar 21, 2015·Biochimica Et Biophysica Acta·F CorneliusS J D Karlish
Sep 22, 2012·Biochimica Et Biophysica Acta·Armando J de Jesus, Toby W Allen
Jan 10, 2012·Chemistry and Physics of Lipids·Thomas GrögerElmar J Prenner
Sep 2, 1999·Experimental Eye Research·J ZengD Borchman
Feb 1, 2008·Biophysical Journal·Derek Marsh
Apr 9, 2015·Frontiers in Physiology·Dragomir Milovanovic, Reinhard Jahn
Sep 8, 2010·Biophysical Journal·Sivaramakrishnan RamaduraiBert Poolman
Nov 9, 2011·Biochimica Et Biophysica Acta·Libo B LiToby W Allen
Oct 29, 2000·Biophysical Journal·C Nielsen, O S Andersen
Apr 17, 1998·Biophysical Journal·C NielsenO S Andersen
Feb 26, 2008·Biochimica Et Biophysica Acta·Derek Marsh
May 15, 1990·Biochimica Et Biophysica Acta·A Villalobo

❮ Previous
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