PMID: 11344049May 10, 2001Paper

The effect of bone morphogenetic protein-2 expression on the early fate of skeletal muscle-derived cells

Bone
D S MusgraveJohnny Huard

Abstract

The identification of bone morphogenetic proteins (BMPs) has stimulated intense interest in BMP delivery approaches. Ex vivo BMP-2 gene delivery has recently been described using skeletal muscle-derived cells. Skeletal muscle-derived cells, because of proven efficient transgene delivery and osteocompetence, represent an attractive cell population on which to base ex vivo BMP-2 gene delivery. However, the early in vivo fate of BMP-2-expressing muscle-derived cells is unknown. This study investigates the in vivo effects of BMP-2 secretion on skeletal muscle-derived cells in terms of cell survival and cell differentiation. The first experiment compared survival of BMP-2-expressing cells with control cells during the first 48 h after in vivo implantation. The results demonstrate that BMP-2 secretion did not adversely affect cell survival 8, 24, or 48 h after intramuscular implantation. The second experiment histologically compared the fate of BMP-2-expressing muscle-derived cells to the same cells not expressing BMP-2. The results show that BMP-2 expression prevented in vivo myogenic differentiation and promoted osteogenic differentiation of the transduced cells. This study further supports the existence of osteoprogenitor cells re...Continue Reading

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Citations

Feb 8, 2005·Microsurgery·Brian C CooleyJeffrey M Toth
Jul 11, 2001·Drug Discovery Today·V J. WrightJ Huard
Mar 11, 2004·Tissue Engineering·Kris A Partridge, Richard O C Oreffo
May 29, 2004·Tissue Engineering·Kevin C HicokJeffrey M Gimble
Aug 26, 2009·Tissue Engineering. Part a·Ching-Kuang ChuangYu-Chen Hu
Sep 3, 2002·Spine·Howard SeehermanRebecca Li
Jun 26, 2002·Annals of the New York Academy of Sciences·W T Godbey, Anthony Atala
May 25, 2006·Journal of Orthopaedic Science : Official Journal of the Japanese Orthopaedic Association·Wei-Jia ChenYukihide Iwamoto
Mar 14, 2009·Differentiation; Research in Biological Diversity·Aaron SchindelerDavid G Little
Oct 5, 2007·Biomaterials·Arvydas Usas, Johnny Huard
Jan 22, 2005·Veterinary Surgery : VS·Louise L SouthwoodC Wayne McIlwraith
Mar 19, 2016·Annals of Biomedical Engineering·A Nazempour, B J Van Wie
Jan 1, 2004·IEEE Engineering in Medicine and Biology Magazine : the Quarterly Magazine of the Engineering in Medicine & Biology Society·Michelle D KofronCato T Laurencin
Apr 7, 2009·Expert Opinion on Biological Therapy·Satheesh ElangovanSrinivas Ayilavarapu
Aug 25, 2006·Biomaterials·Jennifer E PhillipsAndrés J García
Aug 6, 2002·Molecular Therapy : the Journal of the American Society of Gene Therapy·Vonda WrightJohnny Huard
Feb 25, 2005·Molecular Therapy : the Journal of the American Society of Gene Therapy·Osamu SugiyamaJay R Lieberman
Mar 23, 2005·Pathologie-biologie·M-C DegatD Logeart-Avramoglou
Feb 1, 2007·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Mario PescatoriEnzo Ricci
Oct 24, 2002·Clinical Orthopaedics and Related Research·Brett H YoungJohnny Huard
Jun 15, 2018·Molecular Medicine Reports·Wei LuYuyan Zhao
Aug 20, 2003·The Journal of Bone and Joint Surgery. American Volume·Howard SeehermanJohn Wozney

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