The effect of bovine parathyroid hormone withdrawal on MC3T3-E1 cell proliferation and phosphorus metabolism

PloS One
Shuangxin LiuWei Shi

Abstract

Hypocalcemia and hypophosphatemia are common complications after parathyroidectomy (PTX). Sudden removal of high circulating levels of parathyroid hormone (PTH) causes decreased osteoclastic resorption resulting in a decreased bone remodeling space. These phenomena are likely due to an increased influx of calcium and phosphorus into bone. However, there are currently no data to support this hypothesis. In this study, we found that PTX significantly reduced levels of PTH, calcium and phosphate. Compared with preoperative levels, after 1 year, postoperative PTH, calcium and phosphate levels were 295.6 ± 173.7 pg/mL (P < 0.05), 86.62 ± 15.98 mg/dL (P < 0.05) and 5.56 ± 2.03 mg/dL (P < 0.05), respectively. We investigated continuous bovine PTH administration as well as withdrawal of bovine PTH stimulation in the mouse osteoblast precursor cell line MC3T3-E1. MC3T3-E1 cells were cultured with continuous bovine PTH treatment for 20 days or with transient bovine PTH treatment for 10 days. High doses of continuous bovine PTH exposure strongly reduced cell proliferation, alkaline phosphatase activity and the number of mineralized calcium nodules. However, withdrawal of bovine PTH (100 ng/mL) significantly increased the number of mineral...Continue Reading

References

Apr 1, 1988·The American Journal of Medicine·A R Brasier, S R Nussbaum
May 1, 1997·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·D N Cruz, M A Perazella
Apr 25, 2000·American Journal of Nephrology·M GallieniD Brancaccio
Jan 23, 2003·Journal of the American Society of Nephrology : JASN·Claus P SchmittFranz Schaefer
Oct 2, 2003·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·Aiji YajimaOsamu Otsubo
Dec 28, 2004·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Yu-Hsiung WangDavid W Rowe
Feb 9, 2005·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·C P SchmittF Schaefer
Nov 19, 2005·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Geertje van der HorstMarcel Karperien
Jan 25, 2007·Seminars in Dialysis·Eric S Lazar, Nicole Stankus
Feb 20, 2007·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Aiji YajimaOsamu Otsubo
Oct 25, 2007·PloS One·Naohiro KawamuraHiroshi Kawaguchi
Mar 14, 2008·Kidney International·D L Andress
Sep 2, 2008·Nephron. Clinical Practice·Liesbeth ViaeneYves Vanrenterghem
Sep 10, 2008·Current Osteoporosis Reports·Stavroula Kousteni, John P Bilezikian
May 13, 2009·Hemodialysis International·Shih-Mong YehHung-Chun Chen
Jun 10, 2010·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Robert L JilkaStavros C Manolagas
Mar 17, 2011·Advances in Chronic Kidney Disease·Keith A Hruska, Suresh Mathew
May 3, 2011·Molecular and Cellular Biochemistry·Ye TianMing He
May 6, 2011·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Mara J HorwitzAndrew F Stewart
Jul 27, 2011·Current Molecular Pharmacology·Edward M Greenfield
Oct 20, 2011·Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Métabolisme·V RamundoA Faggiano
Nov 17, 2011·Scandinavian Journal of Clinical and Laboratory Investigation·Derya AslanNiklas Rye Jørgensen
Mar 1, 2012·Journal of Electron Microscopy·Tsuneyuki YamamotoNorio Amizuka
Apr 13, 2012·Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Métabolisme·K Kerschan-SchindlP Pietschmann
Jun 8, 2012·Endocrine Journal·Masato YanoHiroshi Kaji
Oct 2, 2012·Journal of Nephrology·Jorge B Cannata-AndíaCarlos Gómez Alonso
Oct 23, 2012·Clinical Nephrology·Linda ShavitItzchak Slotki
Nov 8, 2012·Langenbeck's Archives of Surgery·Lars RolighedPeer Christiansen
Mar 19, 2013·Biochemical Pharmacology·Pedro Esbrit, María José Alcaraz
Jul 25, 2013·International Journal of Nephrology and Renovascular Disease·Joerg LatusMartin Kimmel
Oct 11, 2013·Osteoporosis International : a Journal Established As Result of Cooperation Between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·T SugimotoM Shiraki
Oct 12, 2013·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Sophie NorenstedtInga-Lena Nilsson
Jul 16, 2014·Kidney International Supplements·Shingo FukumaShunichi Fukuhara
Dec 17, 2014·Medical Science Monitor : International Medical Journal of Experimental and Clinical Research·Jiefu SongYunxing Su

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Citations

Apr 5, 2017·Current Opinion in Nephrology and Hypertension·Nishank Jain, Robert F Reilly
Feb 13, 2019·Asian-Australasian Journal of Animal Sciences·Xiaoyun ShenKangning Xiong

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biopsies
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