The effect of Ca(2+)-calmodulin-dependent protein kinase II on cardiac excitation-contraction coupling in ferret ventricular myocytes

The Journal of Physiology
L LiDonald M Bers

Abstract

1. The effect of Ca(2+)-calmodulin-dependent protein kinase II (CaMKII) on excitation-contraction coupling (E-C coupling) was studied in intact ferret cardiac myocytes using the selective inhibitor KN-93, KN-93 decreased steady-state (SS) twitch [Ca2+]i (by 51%), resting Ca2+ spark frequency (by 88%) and SS sarcoplasmic reticulum (SR) Ca2+ content evaluated by eaffeine application (by 37.5%). 2. Increasing extracellular Ca2+ concentration ([Ca2+]o) to 5 mM in KN-93 restored SR Ca2+ load and Ca2+ spark frequency towards that in control (2 mM Ca2+o), but SS twitch [Ca2+]i was still significantly depressed by KN-93. 3. KN-93 decreased Ca2+ transient amplitude of SS twitches much more strongly than the amplitude of post-rest (PR) twitches. In the control, the time constant (Tau) of [Ca2+]i decline of SS twitches was faster than that for PR twitches. This stimulation-dependent acceleration of [Ca2+]i decline was abolished by KN-93. 4. Voltage-clamp experiments demonstrated that KN-93 significantly inhibited sarcolemmal L-type Ca2+ current (ICa) during repetitive pulses by slowing recovery from inactivation. This may explain the preferential action of KN-93 to suppress SS vs. PR twitches. 5. In KN-93, even when both ICa and SR Ca2+ l...Continue Reading

References

Jun 1, 1992·Cell Calcium·H SchulmanT Meyer
Aug 1, 1992·Biophysical Journal·M D Stern
Jan 1, 1991·Journal of Biochemistry·T TakasagoM Shigekawa
Aug 1, 1988·The Journal of General Physiology·R Horn, A Marty
Feb 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·M B Kennedy, P Greengard
Sep 15, 1993·Biochemical and Biophysical Research Communications·N MamiyaH Hidaka
May 1, 1995·The American Journal of Physiology·J W BassaniD M Bers
Jan 1, 1995·Annual Review of Physiology·A P Braun, H Schulman
Jan 1, 1995·The American Journal of Physiology·D M Bers, J R Berlin
Sep 27, 1994·Proceedings of the National Academy of Sciences of the United States of America·R P XiaoE G Lakatta
Jun 1, 1994·The American Journal of Physiology·R CoronadoD M Vaughan
Apr 1, 1993·Pflügers Archiv : European journal of physiology·A VarroD A Eisner
Dec 1, 1996·Circulation Research·E McCallD M Bers

❮ Previous
Next ❯

Citations

Dec 22, 1999·Biochemical and Biophysical Research Communications·H WatanabeK Ohashi
Jun 1, 2005·Heart Rhythm : the Official Journal of the Heart Rhythm Society·Michelle S C KhooMark E Anderson
May 8, 2001·Trends in Pharmacological Sciences·P TaviH Ruskoaho
Feb 20, 2002·Trends in Cardiovascular Medicine·Dirk Hagemann, Rui-Ping Xiao
Apr 7, 2005·Nature Medicine·Donald M Bers
Oct 24, 2012·Nature Reviews. Cardiology·Changwon KhoRoger J Hajjar
May 19, 2010·Proceedings of the National Academy of Sciences of the United States of America·Alexander KushnirAndrew R Marks
Jul 28, 2010·Proceedings of the National Academy of Sciences of the United States of America·David A EisnerLuigi A Venetucci
Aug 22, 2013·Cardiovascular Research·Daniel A GutierrezErnst Niggli
Apr 1, 2009·Journal of Cardiovascular Pharmacology·Donald M Bers, Eleonora Grandi
Apr 13, 2006·Philosophical Transactions. Series A, Mathematical, Physical, and Engineering Sciences·Gentaro IribeDenis Noble
Jun 26, 2003·The Journal of Physiology·Pasi TaviHåkan Westerblad
Jun 9, 2012·Circulation Research·Paari Dominic SwaminathanMark E Anderson
Nov 11, 2010·Theoretical Biology & Medical Modelling·Abhilash KrishnaJohn W Clark
Nov 20, 2013·Annual Review of Physiology·Donald M Bers
Jun 21, 2005·Proceedings of the National Academy of Sciences of the United States of America·Jin O-UchiSatoshi Kurihara
Feb 28, 2001·Proceedings of the National Academy of Sciences of the United States of America·Y WuM E Anderson
Apr 17, 2014·Pflügers Archiv : European journal of physiology·Yu XieHiroshi Matsuura
Jul 17, 2001·The Journal of Physiology·D Georgakopoulos, D Kass
Jan 16, 2007·Cardiovascular Research·Alicia MattiazziCecilia Mundiña-Weilenmann
Jan 16, 2007·Cardiovascular Research·Martin Vila-PetroffAlicia Mattiazzi
Mar 2, 2010·Journal of Molecular and Cellular Cardiology·Anabelle ChaseClive H Orchard
Jul 25, 2014·Circulation Journal : Official Journal of the Japanese Circulation Society·Hyelim ParkBoyoung Joung
Nov 4, 2009·Journal of Molecular and Cellular Cardiology·Young-Hwan SongWon-Kyung Ho
Nov 8, 2011·Journal of Molecular and Cellular Cardiology·Laetitia PereiraAna M Gómez
Jul 6, 2014·Frontiers in Pharmacology·Donald M Bers, Stefano Morotti
Feb 13, 2007·Journal of Molecular and Cellular Cardiology·Michael GrimmThomas Eschenhagen

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.