PMID: 7019679Nov 1, 1980Paper

The effect of caffeine on cell growth and metabolism of thymidine in Escherichia coli

Mutation Research
I SandlieK Kleppe

Abstract

(1) The influence of caffeine on growth and on the metabolism of thymidine was investigated in various E. coli strains. Caffeine caused filamentous growth in all strains investigated. The caffeine effect was reversible. (2) The incorporation of thymidine into DNA was inhibited by caffeine, and the inhibition was most pronounced with bacterial cultures grown overnight in the presence of caffeine before the addition of thymidine. For cells not pretreated with caffeine the inhibitory effect of caffeine decreased with increasing concentrations of thymidine up to about 1 microM whereafter it remained constant. The effect of thymidine concentration on the inhibition was less for bacteria that had grown overnight in the presence of caffeine than for bacteria not pretreated with caffeine. (3) Caffeine inhibited thymidine kinase, but it had no effect on thymidine phosphorylase or thymidine nucleotide kinases. (4) It is suggested that caffeine interferes with uptake of thymidine, conversion of thymidine to dTTP and the DNA synthesis process itself. Filamentous growth could be the result of the inhibition of DNA synthesis.

References

Jan 1, 1976·Mutation Research·C J ChetsangaV Boyd
Nov 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·M GellertH A Nash
Oct 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·S Wickner
Sep 1, 1975·European Journal of Biochemistry·R Hosono, S Kuno
Apr 1, 1972·Journal of Bacteriology·C F BeckE Thomassen
Nov 1, 1967·Proceedings of the National Academy of Sciences of the United States of America·E P KirbyD A Goldthwait
Jan 1, 1968·Molecular & General Genetics : MGG·G W Grigg
Jun 20, 1967·Biochimica Et Biophysica Acta·A Munch-Petersen
Jan 1, 1966·Journal of General Microbiology·G Weinbaum
Jan 1, 1964·Biochemical and Biophysical Research Communications·W Sauerbier
Dec 1, 1954·Journal of Bacteriology·L E LOVELESST H WEISMAN
Apr 1, 1956·Journal of Bacteriology·D J MASON, D M POWELSON
Jan 1, 1964·Journal of Bacteriology·I N DONESON, D M SHANKEL
Oct 1, 1964·Journal of Chromatography·K RANDERATH, E RANDERATH
Apr 1, 1948·Canadian Journal of Research·S STRUGGER

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Citations

Jan 1, 1992·Research in Experimental Medicine. Zeitschrift Für Die Gesamte Experimentelle Medizin Einschliesslich Experimenteller Chirurgie·Y KanemaruT Nakamoto
Dec 6, 2011·Applied Microbiology and Biotechnology·Sathyanarayana N GummadiNandhini Ashok
Apr 7, 2011·Archives of Pharmacal Research·Alaa M HayallahAbdel Alim M Abdel Alim
Sep 4, 2012·Archives of Pharmacal Research·Alaa K M HayallahAbdel Alim M Abdel Alim
Jun 1, 1982·Chemico-biological Interactions·I Sandlie, K Kleppe
Jun 7, 2008·Canadian Journal of Microbiology·Karina CogoFrancisco Carlos Groppo
Jul 7, 2011·Journal of Global Infectious Diseases·Ali Abdul Hussein S Al-Janabi
Jan 1, 1983·Brain & Development·H TanakaM Arima
Aug 20, 2015·Gut Pathogens·Alyssa K Whitney, Tiffany L Weir
Oct 30, 2016·Metabolic Engineering·Maureen McKeagueChristina D Smolke
Jun 1, 1983·Journal of Cellular Physiology·K L BeethamL J Tolmach
Dec 1, 1982·Journal of Cellular Physiology·K L Beetham, L J Tolmach
Nov 16, 2019·World Journal of Microbiology & Biotechnology·Yusuf SürmeliZeynep Petek Çakar
Oct 27, 2017·Pest Management Science·Jonathan Gressel
Apr 11, 2020·The American Journal of Tropical Medicine and Hygiene·Vanaporn WuthiekanunDirek Limmathurotsakul
May 31, 2018·Molecular Medicine Reports·Mahmud MasalhaAnwar Rayan
Jun 3, 2017·The Journal of Antimicrobial Chemotherapy·A E J YsselH P Steenackers

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