PMID: 3214128Jul 1, 1988Paper

The effect of calibration on the between-laboratory variation of serum fructosamine

Annals of Clinical Biochemistry
R P Hill

Abstract

Human pooled serum from diabetic and non-diabetic subjects, and calibrators containing glycated protein or 1-deoxy-1-morpholinofructose (DMF) in a variety of matrices, were distributed to 10 laboratories. When they used their own assay conditions and calibrators, the inter-laboratory variation was unacceptably high. However, when the pool from diabetic patients was reassayed using a calibrator with an assigned value prepared from freeze-dried human serum, and containing no DMF, inter-laboratory variation was reduced significantly. Inter-laboratory agreement for the pool from non-diabetic subjects remained poor despite recalibration. Recalibration using either serum or albumin based solutions of DMF as calibrator failed to effect any significant reductions in inter-laboratory variation. Secondary calibrators based on a protein matrix with no added DMF are recommended for routine use.

References

Jan 7, 1983·Clinica Chimica Acta; International Journal of Clinical Chemistry·R N JohnsonJ R Baker

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Citations

Nov 15, 2017·The Journal of Bone and Joint Surgery. American Volume·Noam ShohatJavad Parvizi

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