The effect of cationic charge on release of eosinophil mediators

The Journal of Allergy and Clinical Immunology
Darryl J AdamkoGerald J Gleich


In patients with atopic diseases, cationic-charged eosinophil proteins are deposited in inflamed tissues. Although the role of cytokines in cell activation is well established, the presence of cationic-charged tissue can also be an important factor in inflammatory cell function. We sought to determine whether increased cationic charge seen in an atopic microenvironment plays a role in the activation of eosinophils. Human eosinophils were incubated with Sepharose beads coated with cationic or anionic compounds in the presence and absence of a cytokine cocktail (IL-3, IL-5, and GM-CSF) to simulate the milieu of inflammation. Eosinophil peroxidase and eosinophil-derived neurotoxin (EDN) release were compared with eosinophil morphology and expression of CD18, as determined by means of confocal microscopy. Cytokines with positively charged beads caused greater eosinophil peroxidase release (lysine coated, 44.2 nmol/L; compound 48/80, 40.0 nmol/L; or EDN coated, 49.1 nmol/L) than cytokines alone (14.9 nmol/L). Beads coated with heparin, dextran sulfate, and aspartic acid did not show this effect. EDN release was also induced by lysine-coated beads with cytokines (67.1 ng/100 microL) and blocked by heparin. Eosinophil incubation with ...Continue Reading


May 28, 1992·Biochemical Pharmacology·H VliagoftisT C Theoharides
Sep 1, 1995·The Journal of Cell Biology·M S DiamondT A Springer
Apr 1, 1993·The American Review of Respiratory Disease·A J CoyleC G Irvin
Jun 1, 1996·American Journal of Respiratory and Critical Care Medicine·Z DiamantP J Sterk
May 13, 1999·The Journal of Biological Chemistry·D A PlagerG J Gleich
Jun 27, 2000·International Archives of Allergy and Immunology·Y MotegiA Morikawa
Apr 9, 2001·Immunological Reviews·B S Bochner, R P Schleimer
Mar 15, 2002·Current Allergy and Asthma Reports·Darryl AdamkoRedwan Moqbel
Jun 18, 2002·International Archives of Allergy and Immunology·Hiroshi TachimotoBruce S Bochner
Jul 18, 2003·Current Eye Research·S D TrocmeC H Dohlman
Feb 11, 2004·Current Allergy and Asthma Reports·Darryl AdamkoRedwan Moqbel
Sep 4, 2004·Journal of Immunological Methods·Darryl J AdamkoRedwan Moqbel
Dec 4, 2004·Plastic and Reconstructive Surgery·Kayvan T KhiabaniWilliam A Zamboni
Mar 22, 2005·Experimental Cell Research·Clemens FeistritzerChristian J Wiedermann
Mar 31, 2005·Trends in Immunology·Giorgio BertonClifford A Lowell

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