The effect of cationic charge on release of eosinophil mediators

The Journal of Allergy and Clinical Immunology
Darryl J AdamkoGerald J Gleich

Abstract

In patients with atopic diseases, cationic-charged eosinophil proteins are deposited in inflamed tissues. Although the role of cytokines in cell activation is well established, the presence of cationic-charged tissue can also be an important factor in inflammatory cell function. We sought to determine whether increased cationic charge seen in an atopic microenvironment plays a role in the activation of eosinophils. Human eosinophils were incubated with Sepharose beads coated with cationic or anionic compounds in the presence and absence of a cytokine cocktail (IL-3, IL-5, and GM-CSF) to simulate the milieu of inflammation. Eosinophil peroxidase and eosinophil-derived neurotoxin (EDN) release were compared with eosinophil morphology and expression of CD18, as determined by means of confocal microscopy. Cytokines with positively charged beads caused greater eosinophil peroxidase release (lysine coated, 44.2 nmol/L; compound 48/80, 40.0 nmol/L; or EDN coated, 49.1 nmol/L) than cytokines alone (14.9 nmol/L). Beads coated with heparin, dextran sulfate, and aspartic acid did not show this effect. EDN release was also induced by lysine-coated beads with cytokines (67.1 ng/100 microL) and blocked by heparin. Eosinophil incubation with ...Continue Reading

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Citations

Aug 6, 2010·Cell and Tissue Research·Yael Minai-FlemingerFrancesca Levi-Schaffer
Jun 27, 2012·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Colleen S Curran, Paul J Bertics
Mar 14, 2009·The Journal of Allergy and Clinical Immunology·Joshua A BoyceBruce S Bochner
Jan 7, 2011·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Marie CarlsonMaria Lampinen
Sep 27, 2014·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·Y Minai-FlemingerF Levi-Schaffer
Apr 5, 2011·Allergy, Asthma & Immunology Research·Hyun Jung JinHae-Sim Park
May 13, 2008·The Journal of Allergy and Clinical Immunology·Francis DavoineDarryl J Adamko
Jan 29, 2019·Allergy·Fanny LegrandFrancesca Levi-Schaffer
Mar 23, 2021·Arthritis & Rheumatology·Mineyo FukuchiMasami Taniguchi

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