PMID: 3765826Aug 1, 1986Paper

The effect of cerulenin on Moloney murine leukemia virus morphogenesis

Virus Research
I KatohR B Luftig

Abstract

Cerulenin is an antibiotic that interferes with fatty acid synthesis in eukaryotic cells. It had been shown by Schultz and Oroszlan (1983), that murine leukemia virus (MuLV) Pr65gag, the polyprotein precursor to the virion core proteins contains the fatty acid myristate at its NH2 terminus. We showed that when 20 micrograms/ml of cerulenin is added for 3 h to mouse fibroblasts chronically infected with Moloney (M)-MuLV it causes a greater than 4-fold decrease in virus production. This is accompanied by an accumulation of uncleaved Pr65gag in the infected cells. Further, thin-section electron micrographs of cerulenin-treated cells show a 2-fold increase in the number of nascent-budding forms, as well as the appearance of aberrant viral forms at the cell membrane. This suggests that the failure to add myristic acid to Pr65gag prevents their proper assembly into viral particles.

References

May 1, 1983·Journal of Virology·A M Schultz, S Oroszlan
Jan 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·L E HendersonS Oroszlan

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Citations

Dec 6, 1989·Biochimica Et Biophysica Acta·M F Schmidt
May 1, 1994·Trends in Microbiology·R B Luftig, L D Lupo
Nov 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·M L BryantJ I Gordon
Feb 1, 1992·AIDS Research and Human Retroviruses·C Debouck
Jan 1, 1989·Archives of Virology·H R GelderblomG Pauli

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