The effect of chaperonin buffering on protein evolution

Genome Biology and Evolution
Tom A Williams, Mario A Fares


Molecular chaperones are highly conserved and ubiquitous proteins that help other proteins in the cell to fold. Pioneering work by Rutherford and Lindquist suggested that the chaperone Hsp90 could buffer (i.e., suppress) phenotypic variation in its client proteins and that alternate periods of buffering and expression of these variants might be important in adaptive evolution. More recently, Tokuriki and Tawfik presented an explicit mechanism for chaperone-dependent evolution, in which the Escherichia coli chaperonin GroEL facilitated the folding of clients that had accumulated structurally destabilizing but neofunctionalizing mutations in the protein core. But how important an evolutionary force is chaperonin-mediated buffering in nature? Here, we address this question by modeling the per-residue evolutionary rate of the crystallized E. coli proteome, evaluating the relative contributions of chaperonin buffering, functional importance, and structural features such as residue contact density. Previous findings suggest an interaction between codon bias and GroEL in limiting the effects of misfolding errors. Our results suggest that the buffering of deleterious mutations by GroEL increases the evolutionary rate of client proteins...Continue Reading


Jul 20, 1973·Science·C B Anfinsen
Apr 2, 1996·Proceedings of the National Academy of Sciences of the United States of America·N A Moran
May 1, 1996·Molecular Biology and Evolution·J L ThorneD T Jones
Aug 28, 1998·Research in Immunology·E CenciL Romani
Dec 9, 1998·Nature·S L Rutherford, S Lindquist
Dec 11, 1999·Science·C A HutchisonJ C Venter
Mar 11, 2000·Molecular Biology and Evolution·C D BustamanteD L Hartl
May 25, 2002·Nature·Mario A FaresEladio Barrio
Jun 7, 2002·Nature·Christine QueitschS Lindquist
Jun 25, 2002·Journal of Molecular Biology·Xiaojun WangBrian K Shoichet
Jan 31, 2003·Nature·Xue Ming Henry HuangMichael L Roukes
Jan 31, 2003·Nature·Csaba PálLaurence D Hurst
Sep 13, 2003·BMC Bioinformatics·Roman L TatusovDarren A Natale
Mar 24, 2004·Journal of Structural Biology·Philip Wong, Walid A Houry
May 7, 2004·Nature·Markus W CovertBernhard O Palsson
Dec 23, 2004·Molecular Microbiology·Masayuki HashimotoJun-ichi Kato
Jan 14, 2005·Bioinformatics·Federico AbascalDavid Posada
Jul 27, 2005·Proceedings of the National Academy of Sciences of the United States of America·Julian Mintseris, Zhiping Weng
Apr 4, 2006·Proceedings of the National Academy of Sciences of the United States of America·Jesse D BloomFrances H Arnold
Oct 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·M Kimura
May 27, 2006·Molecular Systems Biology·Eugene V Koonin
May 8, 2007·Molecular Biology and Evolution·Ziheng Yang
Aug 19, 2007·Molecular Systems Biology·Jun-ichi Kato, Masayuki Hashimoto
Feb 22, 2008·Proceedings of the National Academy of Sciences of the United States of America·Todd A SangsterS Lindquist
Feb 27, 2008·Biology Direct·Katarzyna Tomala, Ryszard Korona
May 9, 2008·PLoS Computational Biology·Nobuhiko TokurikiDan S Tawfik
Feb 24, 2009·Molecular Biology and Evolution·Gavin C Conant, Peter F Stadler
Apr 11, 2009·Science·Nobuhiko Tokuriki, Dan S Tawfik
May 7, 2009·FEMS Microbiology Reviews·Peter A Lund
Jun 6, 2009·Nature Structural & Molecular Biology·F Ulrich Hartl, Manajit Hayer-Hartl
Jun 6, 2009·Nature·Nobuhiko Tokuriki, Dan S Tawfik
Oct 3, 2009·PLoS Computational Biology·José M Peregrín-AlvarezJohn Parkinson
Oct 22, 2009·Molecular Microbiology·Angela M HirtreiterManajit Hayer-Hartl
Jan 21, 2010·Molecular Systems Biology·Tobias Warnecke, Laurence D Hurst
Apr 3, 2010·The EMBO Journal·Kei FujiwaraHideki Taguchi
Apr 9, 2010·Cold Spring Harbor Symposia on Quantitative Biology·S Lindquist
May 11, 2010·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Gregory W Clark, Elisabeth R M Tillier
May 15, 2010·Molecular Biology and Evolution·Christina Toft, Mario A Fares
Jul 10, 2010·Bioinformatics·Liang-Tsung Huang, M Michael Gromiha


Mar 7, 2013·Nature Reviews. Molecular Cell Biology·Evan T Powers, William E Balch
Nov 22, 2013·Genome Biology and Evolution·Sirintra NakjangT Martin Embley
Jun 12, 2012·Molecular Biology and Evolution·Tobias Warnecke
Oct 17, 2012·Molecular Biology and Evolution·Benoit NabholzJochen B W Wolf
Jun 27, 2014·PLoS Computational Biology·Sebastian Pechmann, Judith Frydman
Dec 17, 2014·Mikrobiologiia·I E Vishniakov, S N Borkhsenius
Feb 16, 2013·Genetics·Jennifer LachowiecChristine Queitsch
Jan 22, 2015·Biophysical Journal·Murat Çetinbaş, Eugene I Shakhnovich
Jun 28, 2011·Trends in Plant Science·Carolin Delker, Marcel Quint
Nov 30, 2010·Current Opinion in Structural Biology·Anne Gershenson, Lila M Gierasch
Oct 1, 2015·Trends in Biochemical Sciences·Manajit Hayer-HartlF Ulrich Hartl
Dec 20, 2011·The FEBS Journal·Ariel AziaAmnon Horovitz
Jun 28, 2015·Molecular Biology and Evolution·Beatriz Sabater-MuñozMario A Fares
Sep 20, 2012·Biochemical Society Transactions·Natalia Sanchez de GrootM Madan Babu
May 28, 2015·Trends in Genetics : TIG·Mario A Fares
Jan 1, 2015·Nature Plants·Thomas HauserManajit Hayer-Hartl
Mar 26, 2015·ELife·Marina RudanAnita Krisko
Sep 24, 2014·Molecular Biology and Evolution·Jennifer LachowiecChristine Queitsch
May 18, 2016·Genome Biology and Evolution·A Samer KadibalbanTal Dagan
Nov 22, 2012·Biochemistry·David Bogumil, Tal Dagan
Aug 27, 2016·Molecular Biology and Evolution·Arti TripathiRaghavan Varadarajan
Apr 22, 2017·Molecular Biology and Evolution·Felix Feyertag, David Alvarez-Ponce
Jan 27, 2017·Annual Review of Plant Biology·Andreas BracherManajit Hayer-Hartl
Oct 24, 2014·F1000Research·Rishi Das RoyDebasis Dash
Jun 1, 2019·FEMS Microbiology Letters·José Aguilar-RodríguezAndreas Wagner
Aug 16, 2016·The Biochemical Journal·Chrisostomos Prodromou
Mar 23, 2017·The ISME Journal·Beatriz Sabater-MuñozMario A Fares
Aug 9, 2016·Genome Biology and Evolution·José Aguilar-RodríguezMario A Fares
May 8, 2020·Functional & Integrative Genomics·Manish Prakash VictorTapash Chandra Ghosh
Jul 13, 2019·Genome Biology and Evolution·David Alvarez-PonceMario A Fares

Methods Mentioned

protein folding

Related Concepts

Bacterial Proteins
Alkalescens-Dispar Group
Mycoplasma putrefaciens
Protein Folding, Globular
Chaperonin Complexes
GroEL Protein
Evolution, Molecular
Escherichia coli Proteins

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Alzheimer's Disease: MS4A

Variants within the membrane-spanning 4-domains subfamily A (MS4A) gene cluster have recently been implicated in Alzheimer's disease in genome-wide association studies. Here is the latest research on Alzheimer's disease and MS4A.

Pediculosis pubis

Pediculosis pubis is a disease caused by a parasitic insect known as Pthirus pubis, which infests human pubic hair, as well as other areas with hair including eye lashes. Here is the latest research.

Rh Isoimmunization

Rh isoimmunization is a potentially preventable condition that occasionally is associated with significant perinatal morbidity or mortality. Discover the latest research on Rh Isoimmunization here.

Genetic Screens in iPSC-derived Brain Cells

Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells. It also follows CRISPR-Cas9 approaches to generating genetic mutants as a means of understanding the effect of genetics on phenotype.

Enzyme Evolution

This feed focuses on molecular models of enzyme evolution and new approaches (such as adaptive laboratory evolution) to metabolic engineering of microorganisms. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Pharmacology of Proteinopathies

This feed focuses on the pharmacology of proteinopathies - diseases in which proteins abnormally aggregate (i.e. Alzheimer’s, Parkinson’s, etc.). Discover the latest research in this field with this feed.

Alignment-free Sequence Analysis Tools

Alignment-free sequence analyses have been applied to problems ranging from whole-genome phylogeny to the classification of protein families, identification of horizontally transferred genes, and detection of recombined sequences. Here is the latest research.