The effect of Chlamydia trachomatis infection on the host cell cytoskeleton and membrane compartments.

Journal of General Microbiology
S CampbellP S Yates

Abstract

Human epithelial cells and the McCoy cell line were infected with Chlamydia trachomatis, serotype E. The organization of the cytoplasm was then studied with probes which stained cytoskeletal components and membrane compartments. The major actin-containing stress fibre bundles were not associated with inclusions due to the peri-basal and peri-apical location of these bundles within the host cell. The cytokeratin network was distorted by the presence of inclusions so that a common basket of these intermediate filaments surrounded both nucleus and peri-nuclear inclusions. The microtubule network was similarly distorted, but the nucleus and inclusion were surrounded by separate rather than joint baskets of tubules. After reversible depolymerization by nocadazole the microtubules in amniotic epithelial cells began to reassemble at the peri-nuclear microtubule-organizing centre, so that independent microtubule networks were rapidly regenerated around the nucleus and inclusion. Mitochondria of amniotic epithelial cells were vitally stained with the fluorescent probe DiOC6 (3,3'-dihexyloxacarbocyanine iodide) after 48 h of infection and found to be widely distributed throughout the host cytoplasm. When the morphology of the Golgi compl...Continue Reading

Citations

Jul 1, 1997·Trends in Microbiology·T HackstadtR A Heinzen
Jul 9, 1999·Biochimica Et Biophysica Acta·R GhidoniA Giuliani
Jul 24, 2004·BMC Infectious Diseases·Wilmore C WebleyElizabeth S Stuart
Jan 14, 2009·Current Opinion in Microbiology·Helen J BettsKenneth A Fields
Oct 8, 2016·Frontiers in Cellular and Infection Microbiology·Punsiri M ColonneDaniel E Voth
May 23, 2017·Frontiers in Cellular and Infection Microbiology·Dhritiman SamantaStacey D Gilk
Dec 28, 2019·International Journal of Molecular Sciences·Liam Caven, Rey A Carabeo

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