The effect of choline deficiency on the activity of a phosphatidylcholine-requiring enzyme: activity and properties of UDP-glucuronyltransferase in choline-deficient rats

Biochemical and Biophysical Research Communications
T D Boyer, D Zakim

Abstract

The effect of choline deficiency on the kinetic properties of the microsomal enzyme UDP-glucuronyltransferase (EC2.4.1.17) was investigated in rats. Animals fed choline-deficient diets, as compared with animals fed a choline-replete diet or standard laboratory chow, showed almost a three-fold increase in enzyme activity when the enzyme was assayed at physiological concentrations of UDP-glucuronic acid (0.25 mM). The increase in activity appeared to be due to an enhanced affinity of the enzyme for UDP-glucuronic acid rather than to an increase in the amount of enzyme. These data indicate that the kinetic properties of tightly bound membrane enzymes are altered by a dietary change that is known to cause liver disease in the rat.

References

Jan 13, 1975·Biochimica Et Biophysica Acta·R SaitoB Lombardi
Feb 1, 1975·Proceedings of the Society for Experimental Biology and Medicine·A J PatekK C Hayes
Sep 1, 1972·The American Journal of Clinical Nutrition·E A PortaW S Hartroft
Mar 1, 1974·Proceedings of the Society for Experimental Biology and Medicine·D H RifenbrickJ L Rabinowitz
Oct 1, 1965·Canadian Journal of Biochemistry·S Mookerjea
Sep 7, 2004·Proceedings of the National Academy of Sciences of the United States of America·Young LeeRoger H Unger

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Citations

Jan 1, 1989·European Journal of Clinical Pharmacology·B CrottyF J Dudley
Apr 1, 1986·The American Journal of Medicine·D Zakim

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