The effect of chronic exposure to fatty acids on gene expression in clonal insulin-producing cells: studies using high density oligonucleotide microarray

Endocrinology
J XiaoK Hermansen

Abstract

Fatty acids affect insulin secretion of pancreatic beta-cells. Investigating gene expression profiles may help to characterize the underlying mechanism. INS-1 cells were cultured with palmitate (0, 50, and 200 microM) for up to 44 d. Insulin secretion and expressions of 8740 genes were studied. We found that basal insulin secretion increased in cells exposed to palmitate. The response to glucose stimulation declined on d 44 in cells cultured at 200 microM palmitate. In response to 50 and 200 microM palmitate exposure, expression was changed in 11 and 99 genes on d 2 and 134 and in 159 genes on d 44, respectively. Genes involved in fatty acid oxidation were up-regulated, whereas those involved in glycolysis were down-regulated with 200 microM palmitate. A suppression of insulin receptor and insulin receptor substate-2 gene expression was found on d 44 in cells cultured at 200 microM palmitate. In conclusion, chronic exposure to low palmitate alters insulin secretion as well as gene expression. The number of genes that changed expression was palmitate dose and exposure time dependent. Randle's fatty acid-glucose cycle seems to be operative on the gene transcription level. A modification of expression of various genes may contribu...Continue Reading

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Citations

May 10, 2007·The Review of Diabetic Studies : RDS·Jianguo ChenKjeld Hermansen
Jul 31, 2007·Biochimica Et Biophysica Acta·Christina VockFrank Döring
Feb 5, 2011·Diabetes/metabolism Research and Reviews·Zhenping LiuKjeld Hermansen
Nov 5, 2002·Biochemical and Biophysical Research Communications·Richard BarryIrene C Green
Feb 8, 2005·Biochemical and Biophysical Research Communications·Christopher D SwagellC Phillip Morris
Jul 25, 2006·Clinical Nutrition : Official Journal of the European Society of Parenteral and Enteral Nutrition·Maria Fernanda Cury-BoaventuraRui Curi
Mar 21, 2003·Metabolism: Clinical and Experimental·P B JeppesenK Hermansen
Jan 12, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Tania RescignoAnna Capasso
Nov 10, 2017·PloS One·Veronica SanchoStefano Del Prato
Jun 28, 2006·Diabetes/metabolism Research and Reviews·Jing HongKjeld Hermansen
Apr 3, 2003·Nature Reviews. Genetics·Michael Müller, Sander Kersten
Oct 6, 2005·American Journal of Physiology. Endocrinology and Metabolism·J HongK Hermansen
Jul 16, 2002·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Steve MohrBertrand H Rihn
May 29, 2003·The Journal of Biological Chemistry·Cynthia L KelpeVincent Poitout
Aug 8, 2018·Molecular & Cellular Proteomics : MCP·Zonghong LiJunjie Hou
Nov 1, 2018·Molecular & Cellular Proteomics : MCP·Zonghong LiJunjie Hou

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