The effect of chronic kidney disease on the urine proteome in the domestic cat (Felis catus)

The Veterinary Journal
E FerlizzaG Isani

Abstract

Chronic kidney disease (CKD) is a major cause of mortality in cats, but sensitive and specific biomarkers for early prediction and monitoring of CKD are currently lacking. The present study aimed to apply proteomic techniques to map the urine proteome of the healthy cat and compare it with the proteome of cats with CKD. Urine samples were collected by cystocentesis from 23 healthy young cats and 17 cats with CKD. One-dimensional sodium-dodecyl-sulfate polyacrylamide gel electrophoresis (1D-SDS-PAGE) was conducted on 4-12% gels. Two-dimensional electrophoresis (2DE) was applied to pooled urine samples from healthy cats (n = 4) and cats with CKD (n = 4), respectively. Sixteen protein bands and 36 spots were cut, trypsin-digested and identified by mass spectrometry. 1D-SDS-PAGE yielded an overall view of the protein profile and the separation of 32 ± 6 protein bands in the urine of healthy cats, while CKD cats showed significantly fewer bands (P < 0.01). 2-DE was essential in fractionation of the complex urine proteome, producing a reference map that included 20 proteins. Cauxin was the most abundant protein in urine of healthy cats. Several protease inhibitors and transport proteins that derive from plasma were also identified, i...Continue Reading

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Citations

Oct 27, 2016·The Journal of Small Animal Practice·R E JepsonP B Munroe
Oct 23, 2016·Animal Reproduction Science·Gloria IsaniCarolina Castagnetti
Mar 5, 2016·Journal of Feline Medicine and Surgery·Andrew H SparkesJessica Quimby
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Apr 11, 2021·Journal of Proteomics·André M AlmeidaMaya Zachut

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