The effect of colcemid on the structure and secretory activity of ameloblasts in the rat incisor as shown by radioautography after injection of 3H-proline

The Anatomical Record
A Karim, H Warshawsky

Abstract

Enamel secretion by ameloblasts was investigated in the incisors of 100 gm normal and colcemid-injected male rats. Morphological studies were done on rats given a single intraperitoneal injection of 0.1 mg (1.25 mM) of colcemid and sacrified 1 to 4 hours after injection. Protein synthesis and secretion were investigated with radioautography in normal and colcemid-treated rats injected with 3H-proline and sacrificed at intervals between 0.5 and 3.5 hours after injection. Colcemid was injected 0.5 hours prior to 3H-proline in each experimental rat. Electron microscopic examination revealed several morphological alterations between 1 and 4 hours after injection of colcemid. These changes included fragmentation of the normally elongated rough endoplasmic reticulum into shorter profiles; a disorganization of the normally tubular configuration of the Golgi apparatus into a number of seples and profiles of smooth endoplasmic reticulum from Tomes' processes; and the accumulation of secretion granules at the mature face of the Golgi stacks, as well as in the infranuclear cytoplasm where thye are normally not found. Radioautography revealed that protein synthesis by the rough endoplasmic reticulum had continued in colcemid-altered amelob...Continue Reading

References

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Citations

Apr 1, 1987·Cell and Tissue Research·T Sasaki, P R Garant
Nov 9, 2002·Anatomical Science International·Sumio Nishikawa
Feb 19, 2000·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·B C SorkinZ Skobe
Sep 1, 1984·Journal of Dental Research·S TakumaT Yanagisawa
Oct 1, 1983·The Anatomical Record·S Nishikawa, H Kitamura
Dec 1, 1984·The Anatomical Record·M D McKee, H Warshawsky
Dec 1, 1987·Advances in Dental Research·P R GarantP E Colflesh
Dec 1, 1987·Advances in Dental Research·T SasakiP R Garant
Jan 1, 1985·Archives of Oral Biology·S Nishikawa, H Kitamura
Jul 29, 2008·Archives of Oral Biology·H Lesot, A H Brook

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