PMID: 6165356Nov 15, 1980Paper

The effect of complex-formation with polyanions on the redox properties of cytochrome c

The Biochemical Journal
L C Petersen, R P Cox

Abstract

1. The stable complex formed between mammalian cytochrome c and phosvitin at low ionic strength was studied by partition in an aqueous two-phase system. Oxidized cytochrome c binds to phosvitin with a higher affinity than reduced cytochrome c. The difference was equivalent to a decrease of the redox potential by 22 mV on binding. 2. Complex-formation with phosvitin strongly inhibited the reaction of cytochrome c with reagents that react as negatively charged species, such as ascorbate, dithionite, ferricyanide and tetrachlorobenzoquinol. Reaction with uncharged reagents such as NNN'N'-tetramethylphenylenediamine and the reduced form of the N-methylphenazonium ion (present as the methylsulphate) was little affected by complex-formation, whereas oxidation of the reduced cytochrome by the positively charged tris-(phenanthroline)cobalt(III) ion was greatly stimulated. 3. A similar pattern of inhibition and stimulation of reaction rates was observed when phosvitin was replaced by other macromolecular polyanions such as dextran sulphate and heparin, indicating that the results were a general property of complex-formation with polyanions. A weaker but qualitatively similar effect was observed on addition of inositol hexaphosphate and ...Continue Reading

Citations

Feb 13, 2001·Kidney International. Supplement·S SelaB Kristal
Aug 31, 2007·Thrombosis Research·Edward Young
Mar 13, 2014·PloS One·Evgeny R GalimovPeter M Chumakov
Apr 19, 1982·Biochimica Et Biophysica Acta·S P Brooks, P Nicholls
Aug 15, 1985·Archives of Biochemistry and Biophysics·T YoshimuraK Aki
Jan 6, 2007·Organic & Biomolecular Chemistry·Andrew J WilsonAndrew D Hamilton
Feb 1, 1985·European Journal of Biochemistry·A L MargolinV A Kabanov
Dec 13, 2006·Angewandte Chemie·Koji Kano, Yoshiyuki Ishida
Mar 15, 2002·Chemical Record : an Official Publication of the Chemical Society of Japan ... [et Al.]·S Hirota, O Yamauchi
Dec 1, 2009·Chemistry : a European Journal·James MuldoonAndrew J Wilson
Jan 7, 2011·American Journal of Physiology. Cell Physiology·Boris F KrasnikovDmitry B Zorov
Mar 5, 2003·Biochimica Et Biophysica Acta·Olga V MarkovaVladimir P Skulachev
May 20, 1992·Biochimica Et Biophysica Acta·M AntalíkA Kuchár
Jun 1, 1986·Analytical Biochemistry·H Walter, G Johansson
Feb 24, 2007·Langmuir : the ACS Journal of Surfaces and Colloids·Britto S SandanarajS Thayumanavan
Nov 6, 2021·Science Advances·Alonso Gamero-QuijanoMicheál D Scanlon

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