The effect of compounds altering the cAMP level on reversing the 2-cell block induced by hypoxanthine in mouse embryos in vitro

European Journal of Obstetrics, Gynecology, and Reproductive Biology
D LoutradisA A Kiessling

Abstract

The possibility of reversing the hypoxanthine induced 2-cell block in mouse embryos when cultured in conditions supplemented with compounds that increase (FSH, hMG, IBMX, hCG) or inhibit (GnRH-analogue) cAMP was assessed. When embryos were cultured in Ham's F-10 without hypoxanthine supplemented with each of the above compounds, no inhibition of blastocyst development was observed. Embryos were then cultured in Ham's F-10 with hypoxanthine supplemented again with each compound. For the addition of GnRH-analogue or FSH, the rate of blastocyst formation was comparable with that of the control medium with hypoxanthine alone. Instead, the addition of IBMX or hMG reversed the induced block. There was no reversible effect for the addition of 2 micrograms/ml hCG while the latter was observed with higher doses. The results from GnRH-analogue and IBMX addition show that, contrary to what was found for oocytes, stimulation of cAMP reverses the hypoxanthine-induced block in mouse embryos. FSH and hCG also had effects opposite to those observed for oocytes. It is unknown why hMG (FSH + LH) reverses the block. A lower cAMP degradation rate resulting in a higher cAMP level is a possible explanation. Our results provide further evidence that ...Continue Reading

References

Nov 1, 1990·Journal of Reproduction and Fertility·A NureddinA A Kiessling
Feb 1, 1989·Journal of in Vitro Fertilization and Embryo Transfer : IVF·M JinnoG D Hodgen
Jan 1, 1988·Annals of the New York Academy of Sciences·N Dekel
Jan 1, 1988·Annals of the New York Academy of Sciences·S SuzukiR Iizuka
Sep 1, 1987·Biology of Reproduction·D LoutradisA A Kiessling
Nov 1, 1968·Journal of Reproduction and Fertility·W K Whitten, J D Biggers
Sep 30, 1982·Nature·A Muggleton-HarrisL Wilson
Jul 1, 1981·Journal of Reproduction and Fertility·A McLaren
Sep 1, 1980·Biology of Reproduction·J G Shire, W K Whitten
May 1, 1993·Human Reproduction·J Törnell, T Hillensjö

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Citations

Feb 20, 2007·Annals of the New York Academy of Sciences·Dimitris LoutradisAristidis Antsaklis
Feb 20, 2007·Annals of the New York Academy of Sciences·Panagiotis BeretsosAris Antsaklis
Apr 12, 2005·Reproductive Biomedicine Online·Erasmia KiapekouAntonis Makrigiannakis

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