The effect of concentration on the binding of compound 48/80 to rat mast cells: a fluorescence microscopy study

Immunopharmacology
M J Ortner, C F Chignell

Abstract

A fluorescent analog of the chemical histamine liberator, compound 48/80, has been synthesized by the covalent attachment of rhodamine to the 48/80 polymer (R-48/80). The histamine liberating characteristics of this analog were similar to those of the parent compound. The binding characteristics of R-48/80 to rat peritoneal mast cells were then studied using fluorescence microscopy. At concentrations that caused minimal secretory stimulation (less than 1.0 microgram/ml), R-48/80 bound to the mast cell surface in a diffuse manner, with no indication of patching or capping. When the cells were incubated at higher concentrations, where non-cytotoxic histamine secretion was stimulated, the drug bound heavily to the exposed granules, but not to unexposed granules or other cell organelles. At cytotoxic concentrations, R-48/80 caused extensive cell clumping, with the drug bound to masses of cell debris and released granules. Therefore, although R-48/80 binds initially to the cell membrane, its primary binding site at concentrations that induce secretion becomes the mast cell granule. The properties of these granules should thus be considered when studying the binding of compound 48/80 or other cationic drugs to rat peritoneal mast cells.

References

Apr 15, 1975·Life Sciences·A Goth, A R Johnson
Jun 30, 1975·Annals of the New York Academy of Sciences·T Ishizaka, K Ishizaka
May 17, 1979·Nature·T W Martin, D Lagunoff
Oct 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·F HirataF T Crews
Jan 1, 1975·International Archives of Allergy and Applied Immunology·D C MorrisonP M Henson
Oct 1, 1979·The Journal of Experimental Medicine·D A KennerlyC W Parker

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Citations

Sep 24, 1985·European Journal of Pharmacology·Y KoibuchiK Tomita
Dec 12, 2007·Bioscience, Biotechnology, and Biochemistry·Eun Kyoung KimNak-Won Sohn
May 27, 2009·International Journal of Pharmaceutics·F A Oyarzun-AmpueroM J Alonso
Nov 24, 2011·Macromolecular Bioscience·Felipe A Oyarzun-AmpueroDolores Torres
Jul 19, 2006·Journal of Ethnopharmacology·Hellen K StulzerValfredo Schlemper
Aug 1, 1987·Acta Ophthalmologica·M R AllansmithK J Bloch

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