The effect of condensed tannins in Lotus pedunculatus on the solubilization and degradation of ribulose-1,5-bisphosphate carboxylase (EC 4.1.1.39; Rubisco) protein in the rumen and the sites of Rubisco digestion

The British Journal of Nutrition
W C McNabbT N Barry

Abstract

Three experiments were undertaken to determine the effect of condensed tannin (CT) in Lotus pedunculatus (45-55 g extractable CT/kg DM) on the digestion of the principal leaf protein, ribulose-1,5-bisphosphate carboxylase (EC 4.1.1.39; Rubisco; fraction 1 leaf protein). In two of the experiments Lotus pedunculatus was fed to sheep, with one group receiving a continuous intraruminal infusion (per fistulum) of PEG (molecular weight 3500) to bind and inactivate the CT (PEG group). The other group, which did not receive PEG, was termed the control sheep (CT acting). Expt 3 involved in vitro incubations of Lotus pedunculatus in buffered rumen fluid, with and without PEG added. In all experiments the results have been interpreted in terms of the effects of CT on Rubisco solubilization and degradation. Disappearance of N and Rubisco from Lotus pedunculatus suspended in polyester bags in the rumen was used as a measure of solubilization. Degradation was defined as the disappearance of Rubisco from in vitro incubations of Lotus pedunculatus in rumen fluid. In Expt 1, CT reduced the digestion of Rubisco in the rumen from 0.96 to 0.72 of intake (P < 0.01). Rubisco digestion in the small intestine was 0.27 of intake in control sheep and 0....Continue Reading

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Citations

Sep 26, 2003·The Proceedings of the Nutrition Society·Garry C Waghorn, Warren C McNabb
Nov 27, 2014·Frontiers in Plant Science·Aidyn Mouradov, German Spangenberg
Jul 25, 2018·Journal of Animal Physiology and Animal Nutrition·Byeng Ryel Min, Sandra Solaiman
Mar 29, 2011·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Elsa LamyElvira Sales-Baptista
Sep 4, 2010·Journal of the Science of Food and Agriculture·Amlan K Patra, Jyotisna Saxena
May 1, 2007·Journal of Animal Science·K A BeaucheminT A McAllister
Sep 4, 2008·Journal of Animal Science·J RogosicJ Pavlicevic
Jun 27, 2019·Journal of Agricultural and Food Chemistry·Marissa B RoldanChristine R Voisey
Mar 21, 2002·Journal of Agricultural and Food Chemistry·Allison F WrenLeslie Norris
Nov 29, 2011·Plant Science : an International Journal of Experimental Plant Biology·Francisco J EscarayOscar A Ruiz

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