PMID: 11899749Mar 20, 2002Paper

The effect of Condrosulf (sodium chondroitin sulfate) on proteoglycan synthesis by human osteoarthritic an bovine juvenile articular cartilage chondrocytes--an in vitro study

Acta medica Austriaca
K BobaczW B Graninger

Abstract

Sodiumchondroitinsulfate, Condrosulf, is used in osteoarthritis therapy and belongs to the group of symptomatic slow-acting drugs for osteoarthritis. The aim of this study was to investigate the effects of Condrosulf on total proteoglycan synthesis and cell proliferation in human osteoarthritis and healthy juvenile bovine chondrocytes in vitro. Chondrocytes were grown as monolayers and stimulated for 7 (human cartilage), or 4, 8 and 12 days (bovine cartilage) with different concentrations of Condrosulf (100 micrograms/ml, 500 micrograms/ml, 1000 micrograms/ml, 2500 micrograms/ml and 5000 micrograms/ml). Proteoglycan synthesis was measured by [35S]Sulfate incorporation. The cell proliferation rate was determined using a [3H]Thymidin assay. The expression of the cartilage markers aggrecan and collagen type II was assessed by Northern blot analysis. We show that the incubation with Condrosulf did not affect proteoglycan synthesis neither in osteoarthritis, nor in healthy chondrocytes under the present culture conditions. Cell proliferation rate was also not increased by Condrosulf stimulation. The results of the Northern blot assays demonstrated a dose-dependent down regulation of aggrecan expression on mRNA level. These data indi...Continue Reading

References

Apr 1, 1992·Annals of Internal Medicine·D T FelsonJ J Anderson
Dec 1, 1990·Seminars in Arthritis and Rheumatism·T Hardingham, M Bayliss
Aug 16, 1997·Lancet·P Creamer, M C Hochberg
Apr 3, 1998·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·L ErlacherF P Luyten
Sep 23, 1998·Osteoarthritis and Cartilage·F RoncaG Ronca

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