PMID: 9179567May 1, 1997Paper

The effect of cromakalim on intracellular [Ca2+] in isolated rat skeletal muscle during fatigue and metabolic blockade

Experimental Physiology
F L Burton, G L Smith

Abstract

The effects of the ATP-sensitive K+ channel (KATP channel) opener cromakalim on excitation-contraction (E-C) coupling were studied in skeletal muscle during fatiguing and non-fatiguing activity. Intracellular calcium concentration ([Ca2+]i) was monitored using the fluorescent indicator fura-2 in isolated single skeletal muscle fibres enzymatically dissociated from rat flexor digitorum brevis. A protocol of tetanic stimulation (50 Hz for 300 ms) with progressively shorter durations between tetani was used to induce E-C coupling failure in these cells. Cromakalim (100-800 microM) had little effect on peak [Ca2+]i during twitch and non-fatiguing tetanic stimulation. However, with 0.4 s between tetani, 100 microM cromakalim decreased peak tetanic [Ca2+]i from 1.47 +/- 0.11 microM to 8.35 +/- 55 nM, but did not affect resting [Ca2+]i (control, 220 +/- 40 nM; with cromakalim, 171 +/- 33 nM). Cyanide (2 mM) decreased tetanic [Ca2+]i and increased resting [Ca2+]i during the stimulus protocol; with 0.4 s between tetani, peak [Ca2+]i was 820 +/- 50 nM and resting [Ca2+]i was 443 +/- 32 nM. The ability of cromakalim to inhibit E-C coupling was enhanced by the presence of cyanide. Complete blockade of metabolism by cyanide and iodoactetate...Continue Reading

Citations

May 1, 2016·Experimental Eye Research·Uttio Roy ChowdhuryMichael P Fautsch
Aug 15, 2003·American Journal of Physiology. Cell Physiology·B GongJ M Renaud
Jun 19, 2001·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·W MatarJ M Renaud
Oct 13, 2000·American Journal of Physiology. Cell Physiology·B GongJ M Renaud
Feb 7, 2001·American Journal of Physiology. Cell Physiology·N G MacFarlaneG L Smith
Feb 5, 2003·Progress in Biophysics and Molecular Biology·Susumu Seino, Takashi Miki
May 26, 2012·Journal of Cell Science·Brian R MacIntoshJean-Marc Renaud

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