The effect of curative resection on fecal microbiota in patients with colorectal cancer: a prospective pilot study.

Annals of Surgical Treatment and Research
Sung Sil ParkJae Hwan Oh

Abstract

Although many studies have evaluated the association between intestinal microorganisms and the risk of colorectal cancer (CRC), only a few studies have investigated the changes in microorganisms following curative treatment for CRC. The current study analyzed changes in intestinal microbiota following curative surgery in CRC patients. Stool samples were collected before and 6 months after surgery, from 11 patients with clinical stage III CRC, who underwent curative surgery between May 2017 and June 2017. Next, 16S rRNA gene sequencing was performed. Operational taxonomic units (OTUs) and alpha diversity were evaluated using the Shannon index. The bacterial compositions of the stools were analyzed according to taxonomic rank at genus and phylum levels. OTUs and alpha diversity were significantly decreased following surgery (P < 0.001 and P = 0.019, respectively). The compositions of several bacterial taxa changed after surgery. At genus level, proportions of pathogens such as Campylobacter, Fusobacterium, Haemophilus, Porphyromonas, and Prevotella, decreased after surgery (adjusted P < 0.05). At phylum level, the proportion of Fusobacteria decreased after surgery (adjusted P < 0.001). Significant changes in intestinal microbial ...Continue Reading

References

May 16, 2009·Current Pharmaceutical Design·Ian R Rowland
Apr 13, 2010·Nature Methods·J Gregory CaporasoRob Knight
Sep 18, 2010·Proceedings of the National Academy of Sciences of the United States of America·Les Dethlefsen, David A Relman
Dec 15, 2010·Nature Reviews. Immunology·Maya Saleh, Giorgio Trinchieri
Sep 10, 2011·Bioinformatics·Tanja Magoč, Steven L Salzberg
Jan 18, 2013·The New England Journal of Medicine·Awen M Gallimore, Andrew Godkin
Feb 12, 2013·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Qingchao ZhuHuanlong Qin
Apr 5, 2013·The American Journal of Clinical Nutrition·Hui-Min ChenJing-Yuan Fang
Nov 30, 2014·Molecular Systems Biology·Georg ZellerPeer Bork
Mar 12, 2015·Nature Communications·Qiang FengJun Wang
Oct 31, 2015·Nature Communications·Geicho NakatsuJoseph J Y Sung
Mar 26, 2016·PloS One·Rashmi SinhaJames J Goedert
Jan 1, 2017·Microbiome·Brandilyn A PetersJiyoung Ahn
Jan 13, 2017·Genes & Diseases·Jun Sun, Ikuko Kato
Nov 18, 2017·Microbiome·Marc A SzePatrick D Schloss
Dec 6, 2018·Frontiers in Microbiology·Jing CongXiaochun Zhang
Dec 15, 2018·Environmental Microbiology·Ye JinYunwei Wei

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
electrophoresis
PCA

Software Mentioned

edge R
FLASH
SAS
QIIME

Related Concepts

Related Feeds

Bacteriotherapy (ASM)

Bacteriotherapy, also known as fecal transplantation, involves the transfer of stool from a healthy donor into the gastrointestinal tract for the purpose of treating recurrent C. difficile colitis. Here is the latest on bacteriotherapy.

Bacteriotherapy

Bacteriotherapy, also known as fecal transplantation, involves the transfer of stool from a healthy donor into the gastrointestinal tract for the purpose of treating recurrent C. difficile colitis. Here is the latest on bacteriotherapy.