PMID: 8938369Feb 1, 1996Paper

The effect of cyclosporine in gut ischemic injury: a computerized morphometric and enzymatic analysis

Journal of Pediatric Surgery
R N PuglisiT V Whalen

Abstract

Gut ischemia has been implicated in the pathogenesis of necrotizing enterocolitis. Cyclosporine A (CSA), a potent immunosuppressant, attenuates immune/inflammatory cellular reactions. CSA also might be useful for inhibiting cellular immune responses involved in tissue ischemia/reperfusion injury. The authors hypothesized that CSA would attenuate inflammatory cellular changes associated with gut ischemic injury and that these effects could be quantified by computerized morphometry. Twenty Sprague-Dawley rats underwent 60 minutes of gut ischemia by vascular occlusion of the superior mesenteric vessels. After 1 hour of reperfusion, the ischemic small bowel was harvested for histopathological examination and computerized morphometry, as well as xanthine oxidase (XO, U/mg protein) and maltase (MALT, mmol/L substrate degraded/min/mg protein) assays. CSA (5 mg/kg/d subcutaneously) was given to experimental animals (CSA, n = 10) for 5 days before ischemia, and vehicle was given to controls (CON, n = 10). The computer morphometric parameters studied were: surface index (SI, mucosal surface length per linear unit of intestine), average villous thickness (AVT), and average villous height (AVH). Results are provided in Table 1. The results...Continue Reading

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Citations

Sep 8, 1998·International Journal of Immunopharmacology·H Farghali, K Masek
May 24, 2007·Tissue Engineering·Kevin M SchusterCharles W Hewitt
Sep 16, 2003·Plastic and Reconstructive Surgery·Sakir UnalIsmail Cinel
Apr 26, 2008·Journal of Clinical Biochemistry and Nutrition·Makoto Sasaki, Takashi Joh
May 10, 2011·Internal and Emergency Medicine·Antonio Di SabatinoGino R Corazza
May 12, 2000·Seminars in Pediatric Surgery·J C Chandler, A Hebra
Nov 26, 2008·Transplantation Reviews·Douglas LinfertHamid Rabb
Feb 24, 2001·Journal of Investigative Medicine : the Official Publication of the American Federation for Clinical Research·J K KoC H Cho
Jun 7, 2003·Colorectal Disease : the Official Journal of the Association of Coloproctology of Great Britain and Ireland·G M HydeB F Warren

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