PMID: 9418732Jan 7, 1998Paper

The effect of cytochalasin D on outflow facility and the trabecular meshwork of the human eye in perfusion organ culture

Investigative Ophthalmology & Visual Science
D H Johnson

Abstract

To determine the effect of cytochalasin D on outflow facility in the human anterior segment, and the histologic changes that accompany the effect. Human anterior segments were studied in perfusion organ culture. The anterior segment from one eye received cytochalasin D, and that from the fellow control eye received vehicle; doses ranged from 0.06 mg/ml to 27.7 mg/ml. The duration of action and the effect of repeated doses were studied, and the accompanying histologic changes were assessed in 12 pairs of anterior segments. Cytochalasin D in concentrations of 0.6 mg/ml and 1.1 mg/ml caused increases in outflow facility of 42% and 37%, respectively (P < 0.05), with a peak effect 2 to 6 hours after infusion and a duration of action of approximately 14 hours. Anterior segments were not responsive to repeated doses (24 hours apart). Compared with the effect of vehicle in control anterior segments, cytochalasin D caused scattered breaks in the inner wall endothelial lining of Schlemm's canal (4.6 +/- 2.5% versus 0.7 +/- 0.6%; P = 0.02; anterior segments fixed during maximum drug effect). No increase in the amount of optically empty space within the juxtacanalicular tissue was seen. Inner wall breaks persisted, even in eyes in which th...Continue Reading

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