The effect of devitalized trabecular bone on the formation of osteochondral tissue-engineered constructs.

Biomaterials
Eric G LimaClark T Hung

Abstract

In the current study, evidence is presented demonstrating that devitalized trabecular bone has an inhibitory effect on in vitro chondral tissue development when used as a base material for the tissue-engineering of osteochondral constructs for cartilage repair. Chondrocyte-seeded agarose hydrogel constructs were cultured alone or attached to an underlying bony base in a chemically defined medium formulation that has been shown to yield engineered cartilaginous tissue with native Young's modulus (E(Y)) and glycosaminoglycan (GAG) content. By day 42 in culture the incorporation of a bony base significantly reduced these properties (E(Y)=87+/-12 kPa, GAG=1.9+/-0.8%ww) compared to the gel-alone group (E(Y)=642+/-97 kPa, GAG=4.6+/-1.4%ww). Similarly, the mechanical and biochemical properties of chondrocyte-seeded agarose constructs were inhibited when co-cultured adjacent to bone (unattached), suggesting that soluble factors rather than direct cell-bone interactions mediate the chondro-inhibitory bone effects. Altering the method of bone preparation, including demineralization, or the timing of bone introduction in co-culture did not ameliorate the effects. In contrast, osteochondral constructs with native cartilage properties (E(Y)...Continue Reading

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Citations

Aug 22, 2015·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Adam B NoverClark T Hung
Jan 5, 2016·Medical Engineering & Physics·Adam B NoverClark T Hung
Feb 21, 2016·Journal of Biomechanics·Adam B NoverClark T Hung
Sep 1, 2015·Acta Biomaterialia·Adam B NoverClark T Hung
Nov 15, 2013·Advanced Materials·Julian ThieleWilhelm T S Huck
May 28, 2013·Computer Methods in Biomechanics and Biomedical Engineering·Jérôme HollensteinDominique P Pioletti
May 23, 2019·Journal of Tissue Engineering and Regenerative Medicine·Benjamin L LarsonLisa E Freed
Mar 3, 2011·Clinical Orthopaedics and Related Research·Prakash JayabalanJames L Cook
Sep 28, 2018·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Yogendra Pratap SinghBiman B Mandal

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