PMID: 6344925Jul 5, 1983Paper

The effect of dexamethasone on pyruvate kinase activity in primary cultures of hepatocytes

Biochimica Et Biophysica Acta
O MiyanagaG L Cottam

Abstract

Pyruvate kinase activity in primary cultures of hepatocytes isolated from a normal rat was maintained at a constant level similar to that found in vivo (14.0 +/- 2.8 units per mg of DNA) for over 6 days when both dexamethasone and insulin were included in the medium. Yet the pyruvate kinase activity decreased 50% when the cells were cultured for 2 days and 4 days, respectively, in the presence of either dexamethasone or insulin alone. A brief, 10 min incubation of hepatocytes in the presence of dexamethasone was sufficient to maintain the enzyme activity of cells subsequently cultured for 4 days in the presence of insulin. The optimal dexamethasone concentration was 1 microM. Three other glucocorticoids were able to maintain the pyruvate kinase activity in cells cultured in medium containing insulin. The presence of the protein synthesis inhibitors, actinomycin D or cyclohexamide in cells cultured in the presence of dexamethasone and insulin resulted in a 25% decrease in the pyruvate kinase activity. Therefore, it is suggested that the synergistic effect of glucocorticoids and insulin to maintain pyruvate kinase activity in primary cultures of hepatocytes is dependent upon the ability of these cells to maintain protein synthesis.

References

Oct 1, 1976·Archives of Biochemistry and Biophysics·E A Kohl, G L Cottam
Apr 28, 1972·Biochemical and Biophysical Research Communications·W R Ingebretsen, S R Wagle
Oct 1, 1967·Proceedings of the National Academy of Sciences of the United States of America·W Kreutner, N D Goldberg
May 13, 1980·Biochemistry·M L Johnson, C M Veneziale
Mar 1, 1980·Analytical Biochemistry·C Labarca, K Paigen
Aug 1, 1980·Archives of Biochemistry and Biophysics·M NaganoG L Cottam
Apr 1, 1980·Archives of Biochemistry and Biophysics·C Cladaras, G L Cottam
Jul 2, 1965·Science·G WeberE A FISHER

Citations

Dec 1, 1989·Neurochemical Research·N WestergaardJ Dich
Jan 1, 1988·The International Journal of Biochemistry·S S WongD C Yeung
Sep 1, 1984·Archives of Biochemistry and Biophysics·C EvansG L Cottam
Mar 15, 1984·Biochemical and Biophysical Research Communications·O MiyanagaG L Cottam
Apr 29, 2009·Journal of Ethnopharmacology·Cheng-Jie ChenMin Li
Jan 1, 1988·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·J DichN Grunnet
Mar 1, 1987·The American Journal of Physiology·S M HutsonL S Jefferson

Related Concepts

Zyderm
Oradexon
Hydrocortisone, (9 beta,10 alpha,11 alpha)-Isomer
Novolin
Liver
Predonine
R-Type Pyruvate Kinase
August Rats
Volon

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Lipidomics & Rhinovirus Infection

Lipidomics can be used to examine the lipid species involved with pathogenic conditions, such as viral associated inflammation. Discovered the latest research on Lipidomics & Rhinovirus Infection.

Glut1 Deficiency

Glut1 deficiency, an autosomal dominant, genetic metabolic disorder associated with a deficiency of GLUT1, the protein that transports glucose across the blood brain barrier, is characterized by mental and motor developmental delays and infantile seizures. Follow the latest research on Glut1 deficiency with this feed.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Alzheimer's Disease: MS4A

Variants within the membrane-spanning 4-domains subfamily A (MS4A) gene cluster have recently been implicated in Alzheimer's disease in genome-wide association studies. Here is the latest research on Alzheimer's disease and MS4A.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Laryngeal Neoplasms

Laryngeal Neoplasms occur in the Larynx and are typically associated with smoking and alcohol consumption. Discover the latest research on Laryngeal Neoplasms here.

Cell Atlas Along the Gut-Brain Axis

Profiling cells along the gut-brain axis at the single cell level will provide unique information for each cell type, a three-dimensional map of how cell types work together to form tissues, and insights into how changes in the map underlie health and disease of the GI system and its crosstalk with the brain. Disocver the latest research on single cell analysis of the gut-brain axis here.