PMID: 3769516Oct 1, 1986Paper

The effect of dialysis of extended ram semen prior to freezing on post-thaw survival and fertility

Cryobiology
M K SchmehlE F Graham

Abstract

The effect of dialysis on extended ram semen prior to cryopreservation was studied. Techniques were developed to improve post-thaw recovery of dialyzed semen and a fertility trial was used to evaluate the viability of dialyzed and frozen semen. Dialysis prior to freezing was shown to increase post-thaw recovery of motile cells and percentage of cells passing through a Sephadex filter. Freezing semen in pellets on dry ice was superior to freezing in French straws. Pellets were thawed in an aluminum thaw block at 42 to 45 degrees C before insemination of progestagen-PMSG synchronized ewes. Double inseminations were made at 12-hr intervals. Natural service of synchronized ewes was also made at 12-hr intervals as a control. There was no significant difference (P greater than 0.05) in fertility between naturally serviced ewes (44.4%) and ewes inseminated with frozen semen (44.7%).

References

Jun 1, 1978·Cryobiology·B K Gustafsson
Nov 1, 1975·Biology of Reproduction·R H Hammerstedt
Mar 1, 1969·Journal of Reproduction and Fertility·P J Quinn, I G White
Jul 1, 1983·Journal of Reproduction and Fertility·J W BaasP Shannon
May 1, 1981·Journal of Reproduction and Fertility·K Bamba, M Sone

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Citations

May 17, 1996·Journal of Chromatography. B, Biomedical Applications·N García-LópezT Muiño-Blanco
Aug 23, 2001·Theriogenology·R Pérez-PéT Muiño-Blanco
Mar 27, 2001·Animal Reproduction Science·M A El-Alamy, R H Foote
Aug 28, 2001·Journal of Chromatography. B, Biomedical Sciences and Applications·R Pérez-PéJ A Cebrián-Pérez
Jul 28, 2010·Reproduction : the Official Journal of the Society for the Study of Fertility·R WaurichK Jewgenow
Jan 29, 2016·Reproduction in Domestic Animals = Zuchthygiene·C KlausK Müller
Apr 17, 2007·Theriogenology·E MartiJ A Cebrián-Pérez
Sep 23, 2008·Reproduction in Domestic Animals = Zuchthygiene·T Muiño-BlancoJ A Cebrián-Pérez
Nov 4, 2000·Biology of Reproduction·B BarriosJ A Cebrián-Pérez
Dec 12, 2001·International Journal of Andrology·R Pérez-PéJ A Cebrián-Pérez
Jan 1, 2014·Journal of Animal Science and Technology·Davendra Kumar, Syed Mohammed Khursheed Naqvi

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