PMID: 702211Oct 1, 1978Paper

The effect of dietary fat or cholesterol and cholic acid on the rate of synthesis of rat liver glucose-6-P dehydrogenase

The Journal of Nutrition
R G Wolfe, D Holten

Abstract

The effect of dietary fat or cholesterol and cholic acid on the rate of synthesis of rat liver glucose-6-P dehydrogenase was measured by isolating hepatocytes from the livers of rats fed diets containing various amounts and types of lipid. Hepatocyte proteins were labeled with [3H]leucine and glucose-6-P dehydrogenase was immunoprecipitated with a specific antiserum. The immunoprecipitates were electrophoresed on sodium dodecyl sulfate-polyacrylamide gels and the radioactivity in the glucose-6-P dehydrogenase subunit was used to calculate the relative rate of synthesis of the enzyme. It is concluded that the activity of glucose-6-P dehydrogenase is primarily regulated by alterations in the rate at which the enzyme is synthesized. Dietary fat may decrease the rate of degradation of glucose-6-P dehydrogenase.

Citations

Jan 2, 2001·The Journal of Biological Chemistry·B Amir-Ahmady, L M Salati
May 26, 2001·Annual Review of Nutrition·L M Salati, B Amir-Ahmady
Aug 31, 2004·The Journal of Nutrition·Lisa M SalatiDeborah L Hodge
May 16, 1984·Biochemical and Biophysical Research Communications·N MorikawaD Holten
Jul 12, 1979·Biochemical and Biophysical Research Communications·R G WolfeD Holten

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