The effect of dietary resistant starch type 2 on the microbiota and markers of gut inflammation in rural Malawi children

Microbiome
M Isabel OrdizMark J Manary

Abstract

Resistant starch (RS) decreases intestinal inflammation in some settings. We tested the hypothesis that gut inflammation will be reduced with dietary supplementation with RS in rural Malawian children. Eighteen stunted 3-5-year-old children were supplemented with 8.5 g/day of RS type 2 for 4 weeks. The fecal samples were analyzed for the microbiota, the microbiome, short chain fatty acids, metabolome, and proteins indicative of inflammation before and after the intervention. Subjects served as their own controls. The consumption of RS changed the composition of the microbiota; at the phylum level Actinobacteria increased, while Firmicutes decreased. Among the most prevalent genera, Lactobacillus was increased and Roseburia, Blautia, and Lachnospiracea incertae sedis were decreased. The Shannon H index at the genus level decreased from 2.02 on the habitual diet and 1.76 after the introduction of RS (P < 0.01). Fecal acetate concentration decreased, and fecal propionate concentration increased after RS administration (-5.2 and 2.0 μmol/g, respectively). Fecal calprotectin increased from 29 ± 69 to 89 ± 49 μg/g (P = 0.003) after RS was given. The lipopolysaccharide biosynthesis pathway was upregulated. Our findings do not support ...Continue Reading

Associated Clinical Trials

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Citations

Mar 5, 2016·Archives of Disease in Childhood·Indi TrehanMark J Manary
Feb 1, 2017·Future Microbiology·Zohreh Tamanai-ShacooriAnne Jolivet-Gougeon
Apr 16, 2017·Applied and Environmental Microbiology·Marie S MøllerMaher Abou Hachem
Mar 26, 2019·Annual Review of Food Science and Technology·Yanbei WuThomas T Y Wang
Jan 31, 2016·Current Opinion in Gastroenterology·Janina A KrumbeckRobert W Hutkins
Jul 19, 2019·Frontiers in Microbiology·Yanan WangGeraint B Rogers
Mar 12, 2016·Current Opinion in Infectious Diseases·Andrew J Prendergast, Paul Kelly
Jun 19, 2020·Journal of Diabetes and Metabolic Disorders·Hanieh-Sadat EjtahedBagher Larijani
Sep 29, 2017·Frontiers in Microbiology·Chiara FerrarioFrancesca Turroni
Aug 12, 2018·Nutrients·Tiehua ZhangChanghui Zhao
Aug 23, 2018·Frontiers in Microbiology·Zhanshan MaWendy Li
Jul 20, 2021·Gut Microbes·Peter A Dobranowski, Alain Stintzi

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Methods Mentioned

BETA
metabolomic profiling
PCR
Profiler
PCA

Software Mentioned

MBLASTX
ChimeraSlayer
DUST
GraphPad Quick Calcs
Vegan package
SPSS
BMTagger
TrimBWAStyle
Mass Profiler Professional
Fasta

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