The effect of different agitation modes on platelet metabolism, thromboxane formation, and alpha-granular release during platelet storage

Transfusion
J WallvikO Akerblom

Abstract

Platelet concentrates (PCs), prepared by plateletpheresis, were stored in aliquots in polyvinylchloride blood bags for 5 days at 22 degrees C under rapid, slow, or no agitation. Nonagitated PCs were also stored in a 98-percent oxygen atmosphere. In nonagitated PCs, pO2, lactate production, and platelet factor 4 (PF 4) concentration increased, whereas the ATP level and pH dropped rapidly. These changes were somewhat minimized in nonagitated PCs stored in oxygen. There was no significant difference between the two agitated groups. The increase in PF 4 correlated inversely to the decrease in ATP: r = -0.91, p less than 0.001, n = 24. The formation of thromboxane B2 (TxB2) after stimulation with arachidonic acid or collagen was significantly higher in slowly agitated PCs on Day 5 than on Day 0 (p less than 0.01). Nonagitated PCs produced lower levels of TxB2 (collagen stimulation) on Day 5 (p less than 0.05). In unstimulated PCs, the levels of TxB2 and ATP were inversely correlated on Day 5 (r = -0.70, p less than 0.001, n = 20). In vivo survival was performed after 72 hours of storage; mean survival (+/- SD) was 6.5 (+/- 0.3) days for nonagitated oxygenated PCs and 6.8 (+/- 0.7) days for agitated PCs. In nonagitated PCs, anaerobic...Continue Reading

Citations

Jun 1, 1992·Transfusion Medicine·J WallvikM Blombäck
Jan 1, 1994·Vox Sanguinis·S G MitchellL K Harding
Nov 26, 2013·Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis·Timori Naghadeh HossienKarimi Gharib
Apr 26, 2011·Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis·Pieter F van der Meer, Dirk de Korte
Jun 18, 2010·Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis·Stephen J WagnerGary Moroff
Nov 26, 2009·Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis·Stephen J Wagner
May 10, 2005·Vox Sanguinis·P F van der MeerUNKNOWN Biomedical Excellence for Safer Transfusion (BEST) Collaborative
May 12, 2006·Transfusion·Erwin F StrasserReinhold Eckstein
May 8, 1994·Transfusion Science·S Holme
May 1, 1993·Thrombosis Research·J Wallvik, A M Suontaka
May 9, 2021·Scientific Reports·Maria-Argyro Karageorgou, Dimosthenis Stamopoulos

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